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Table S1 Characteristics of the Plasmodium falciparum samples collected in Africa and South America, with their geographical coordinates, total sample size and mono-infected sample size.

Table S2 Characteristics of the Plasmodium falciparum samples retrieved from literature, with their geographical coordinates, total sample size and mono-infected sample size.

Table S3 Primers used for first and second PCR.

Table S4 Estimates of genetic differentiation (FST) obtained between pairs of populations and P-value of the test of differentiation for each gene.

Fig. S1 Average nucleotide diversity, Tajima's D, Fu and Li's D* and Fay and Wu's H measured in the P. falciparum populations collected in South America.

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