mec12708-sup-0001-FigureS1-S3.docxWord document167K

Fig. S1 Chen et al.'s 2007 hypothesis of the origin of the causal deletion mutation in the regulatory region of style2.1. Our alternative hypothesis, Figure 4, includes more contemporary phylogenetic (species) designations, and relationships. Figure 4 cladogram is based on a phylogenetic reconstruction using 18 unlinked loci in RAxML; nodes with less support (bootstrap <70, posterior probability <0.95) are depicted as polytomies (Haak, Ballenger and Moyle, in press).

Fig. S2 Floral measurements included as quantitative genetic traits. CD = corolla diameter, AL = stamen length, SL = style length and SE = stigma exsertion.

Fig. S3 Relationship between phenotypic variation in (A) style length and (B) stigma exsertion within S. pimpinellifolium (Y-axis) and altitude (X-axis) at collection site. The two accessions that have deletion variants at StyleD1 are marked with asterisks, reiterating that phenotypic variation is not associated with deletion variation. Instead, for SE there is a significant negative relationship with altitude (log-transformed) (F = 13.24, P < 0.0015) possibly due to variation in pollinator availability and/or reduced population densities at the species range margin. This relationship is marginal for SL (F = 2.98, P = 0.099) and not significant for CD or AL (data not shown). In comparison, SE is marginally correlated with latitude (F = 3.04, P < 0.096), but this relationship is stronger for the other floral traits (CD: F = 7.73, P < 0.0112; AL: F = 15.79, P < 0.0007; SL F = 15.20, P < 0.0008) as has been previously reported for size-related floral traits in this species (Caicedo & Schaal 2004).


Table S1 Species and accession IDs, and trait means for 74 accessions of Solanum.

Table S2 Genotypes at StyleDel1 and StyleDel2 for 74 accessions of Solanum.

Table S3 Principal components analysis (PCA) on four reproductive traits.

Table S4 One-way nested anova results for each of four quantitative floral traits.

Table S5 Physical location of deletion breakpoints in I and S deletion alleles in StyleD1 and StyleD2.

Table S6 Within species associations (S. lycopersicum var. cerasiforme and S. lycopersicum) between StyleD1 alleles and phenotypic variation.

Table S7 Floral trait (SL and SE) and biogeographical data for 24 S. pimpinellifolium accessions.

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