Involvement of Histamine in Endothelium-Dependent Relaxation of Mesenteric Lymphatic Vessels
Article first published online: 10 OCT 2014
© 2014 John Wiley & Sons Ltd
Volume 21, Issue 7, pages 640–648, October 2014
How to Cite
Involvement of histamine in endothelium-dependent relaxation of mesenteric lymphatic vessels. Microcirculation 21: 640–648, 2014., , , , , , , .
- Issue published online: 10 OCT 2014
- Article first published online: 10 OCT 2014
- Accepted manuscript online: 21 APR 2014 09:51AM EST
- Manuscript Accepted: 16 APR 2014
- Manuscript Received: 20 FEB 2014
- National Institutes of Health. Grant Numbers: AG-030578, HL-094269
- Texas A&M University Health Science Center College of Medicine
- mesenteric lymphatic vessels;
- lymphatic endothelium;
The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium-derived histamine relaxes MLVs.
We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by l-NAME (100 μM) or/and histamine production by α-MHD (10 μM). Effectiveness of α-MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine-producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo-morpholino oligos.
We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow-dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells.
This study supports a role for histamine as an EDRF in MLVs.