M. S. and A. C. L. contributed equally to this work.
Gene silencing by H-NS from distal DNA site
Article first published online: 14 SEP 2012
© 2012 Blackwell Publishing Ltd
Volume 86, Issue 3, pages 707–719, November 2012
How to Cite
Shin, M., Lagda, A. C., Lee, J. W., Bhat, A., Rhee, J. H., Kim, J.-S., Takeyasu, K. and Choy, H. E. (2012), Gene silencing by H-NS from distal DNA site. Molecular Microbiology, 86: 707–719. doi: 10.1111/mmi.12012
- Issue published online: 24 OCT 2012
- Article first published online: 14 SEP 2012
- Accepted manuscript online: 24 AUG 2012 03:10AM EST
- Manuscript Accepted: 20 AUG 2012
- MOST. Grant Number: 2007-04213
- Regional Technology Innovation Program of the MOCIE. Grant Number: RT105-01-01
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Korean Research Foundation
In the modern concept of gene regulation, ‘DNA looping’ is the most common underlying mechanism in the interaction between RNA polymerase (RNAP) and transcription factors acting at a distance. This study demonstrates an additional mechanism by which DNA-bound proteins communicate with each other, by analysing the bacterial histone-like nucleoid-structuring protein (H-NS), a general transcriptional silencer. The LEE5 promoter (LEE5p) of enteropathogenic Escherichia coli was used as a model system to investigate the mechanism of H-NS-mediated transcription repression. We found that H-NS represses LEE5p by binding to a cluster of A tracks upstream of −114, followed by spreading to a site at the promoter through the oligomerization of H-NS molecules. At the promoter, the H-NS makes a specific contact with the carboxy terminal domain of the α subunit of RNAP, which prevents the processing of RNAP–promoter complexes into initiation-competent open promoter complexes, thereby regulating LEE5p from distance.