These authors contributed equally to this work.
Research Article
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Spo0A links de novo fatty acid synthesis to sporulation and biofilm development in Bacillus subtilis
Article first published online: 7 DEC 2012
DOI: 10.1111/mmi.12102
© 2012 Blackwell Publishing Ltd
Additional Information
How to Cite
Pedrido, M. E., de Oña, P., Ramirez, W., Leñini, C., Goñi, A. and Grau, R. (2013), Spo0A links de novo fatty acid synthesis to sporulation and biofilm development in Bacillus subtilis. Molecular Microbiology, 87: 348–367. doi: 10.1111/mmi.12102
- †
These authors contributed equally to this work.
Publication History
- Issue published online: 14 JAN 2013
- Article first published online: 7 DEC 2012
- Accepted manuscript online: 21 NOV 2012 10:47AM EST
- Manuscript Accepted: 13 NOV 2012
Funded by
- CONICET (National Research Council of Argentina)
- ANCyT (National Agency of Science and Technology)
- Pew Latin-American Program in Biological Sciences
- Fulbright Committee
- Fundación Antorchas
References
- , , , and (2002) A cytoskeleton-like role for the bacterial cell wall during engulfment of the Bacillus subtilis forespore. Genes Dev 12: 3253–3264.
- , , (2011) Biofilm formation and dispersal in Gram positive bacteria. Curr Opin Biotechnol 22: 172–179.
- , , , and (2007) Thinking about Bacillus subtilis as a multicellular organism. Curr Opin Microbiol 10: 638–643.
- , , , , , and (2003) Characterization of a novel inhibitory feedback of the anti-anti-sigma SpoIIAA on Spo0A activation during development in Bacillus subtilis. Mol Microbiol 47: 1251–1263.
- , , , and (1999) The SpoIIE phosphatase, the sporulation septum and the establishment of forespore-specific transcription in Bacillus subtilis: a reassessment. Mol Microbiol 31: 1407–1415.
- , , , , and (2008) Lipid spirals in Bacillus subtilis and their role in cell division. Mol Microbiol 68: 1315–1327.
- , , , , , , et al. (2003) Multifunctioal acetyl-CoA carboxylase 1 is essential for very long chain fatty acid elongation and embryo development in Arabidopsis. Plant J 33: 75–86.
- , , and (2008) Lipids in the assembly of membrane proteins and the organization of protein supercomplexes: implications for lipid-linked disorders. Subcell Biochem 49: 197–239.
- , , , , and (2001) Fruiting body formation by Bacillus subtilis. Proc Natl Acad Sci USA 98: 11621–11626.
- , , , , and (2006) A major protein component of the Bacillus biofilm matrix. Mol Microbiol 59: 1229–1238.
- , and (2006) Forespore engulfment mediated by a ratchet-like mechanism. Cell 26: 917–928.
- , , , , and (2007) The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis. Cell 131: 1301–1312.
- , , and (2008) SpoIIQ anchors membrane proteins on both sides of the sporulation septum in Bacillus subtilis. J Biol Chem 283: 4975–4982.
- , , , and (2010) Loss of compartmentalization of Sigma E activity need not prevent formation of spores by Bacillus subtilis. J Bacteriol 192: 5616–5624.
- (2008) Beyond the origin. Beneath the surface. Nature 456: 300–303.
- , , , , , , and (2003) Fatty acid lipidic fractions in extracellular polymeric substances of activated sludge flocks. Lipids 38: 1093–1105.
- , , , , and (1990) A forespore checkpoint for mother cell gene expression during development in Bacillus subtilis. Cell 62: 239–250.
- , , and (2003) Rhamnolipid surfactant production affects biofilm architectured in Pseudomonas aeruginosa PAO 1. J Bacteriol 185: 1027–1036.
- , , , and (2012) Vectorial signalling mechanism required for cell-cell communication during sporulation in Bacillus subtilis. Mol Microbiol 83: 261–274.
- , and (2009) Lipid-dependent membrane protein topogenesis. Annu Rev Biochem 78: 515–540.
- , and (2011) Lipid-proteins interactions as determinants of membrane protein structure and function. Biochem Soc Trans 39: 767–774.
- (2003) Transient genetic asymmetry and cell fate in a bacterium. Trends Genet 19: 107–112.
- , and (2001) Differential gene expression governed by chromosomal spatial asymmetry. Cell 107: 339–346.
- , and (2001) Membrane lipid control of cytokinesis. Cell Struct Funct 26: 659–665.
- (2005) Membranes are more mosaic than fluid. Nature 438: 578–580.
- , , , and (2011) Bacterial biofilm shows persistent resistance to liquid wetting and gas penetration. Proc Natl Acad Sci USA 108: 995–1000.
- (2003) Regulation of endospore formation in Bacillus subtilis. Nat Rev Microbiol 1: 117–126.
- , and (2008) A one-sided signal. Science 320: 458–460.
- , , and (1998) The Bacillus SpoIIGA protein is targeted to sites of spore septum formation in a SpoIIE-independent manner. Mol Microbiol 28: 931–943.
- , and (2010) The biofilm matrix. Nat Rev Microbiol 8: 623–633.
- , , , and (1999) Transient gene asymmetry during sporulation and establishment of cell specificity in Bacillus subtilis. Genes Dev 13: 394–399.
- , and (2002) An investigation into the compartmentalization of the sporulation transcription factor σE in Bacillus subtilis. Mol Microbiol 43: 27–38.
- , and (2003) The master regulator Spo0A for entry into sporulation in Bacillus subtilis becomes a cell-specific transcription factor after asymmetric division. Genes Dev 17: 1166–1174.
- , , and (2007) Regulation of fatty acid metabolism in bacteria. Mol Microbiol 66: 829–839.
- , and (2007) The PAR proteins: fundamentals players in animal cell polarization. Dev Cell 13: 609–622.
- , , , , , , et al. (2005) The Bacillus subtilis SinR and RapA developmental regulators are responsible for inhibition of spore development by alcohol. J Bacteriol 187: 2662–2672.
- , and (1993) Regulation of the synthesis of unsaturated fatty acids by growth temperature in Bacillus subtilis. Mol Microbiol 8: 535–542.
- , and (2001) The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis. Mol Microbiol 42: 1199–1209.
- , , , , and (2004) Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis. Mol Microbiol 52: 847–860.
- , and (1989) Checkpoints: controls that ensure the order of cell cycle events. Science 246: 629–634.
- , and (2012) Recent progress in Bacillus subtilis sporulation. FEMS Microbiol Rev 36: 131–148.
- , and (2004) Compartmentalization of gene expression during Bacillus subtilis spore formation. Microbiol Mol Biol Rev 68: 234–262.
- , , , and (2008) Evidences that the Bacillus subtilis SpoIIGA protein is a novel type of signal-transducing aspartic protease. J Biol Chem 283: 15287–15299.
- , and (1992) Coupling between gene expression and DNA synthesis early during development in Bacillus subtilis. Proc Natl Acad Sci USA 89: 8808–8812.
- , , , , , and (2004) Local exposure of phosphatydylethanolamine on the yeast plasma membrane is implicated in cell polarity. Genes Cells 9: 891–903.
- , , and (2003) Membrane fusion. Cell 112: 519–533.
- , , and (1995) Identification of a gene spoIIR, that links the activation of σE to the transcriptional activity of σF during sporulation in Bacillus subtilis. Proc Natl Acad Sci USA 92: 2012–2016.
- , and (2005) Cell population heterogeneity during growth of Bacillus subtilis. Genes Dev 19: 3083–3094.
- , , , , and (1999) Septation, dephosphorylation, and the activation of σF during sporulation in Bacillus subtilis. Genes Dev 13: 1156–1167.
- , , , and (2011) A σW-dependent stress response in Bacillus subtilis that reduces membrane fluidity. Mol Microbiol 81: 69–79.
- , and (2012) BslA (YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms. Mol Microbiol 85: 51–56.
- , , and (2012) The protective layer of a biofilm: a repellent function for a new class of amphiphilic proteins. Mol Microbiol 85: 8–11.
- , , , , , and (2005) Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation. Appl Environ Microbiol 71: 39–45.
- (2005) Lessons from DNA microarray analysis: the gene expression profile of biofilms. Curr Opin Microbiol 8: 222–227.
- (2005) A greasy grip. Nature 438: 569–570.
- , , and (2003) Computational identification of the Spo0A-phosphate regulon that is essential for the cellular differentiation and development in Gram-positive spore-forming bacteria. Nucleic Acids Res 23: 6891–6903.
- , , , and (2006) A LuxS-dependent cell to cell language regulates social behavior and development in Bacillus subtilis. J Bacteriol 188: 4442–4452.
- , and (1995) Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis. Genes Dev 9: 503–508.
- , and (2010) Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis. FEMS Microbiol Rev 34: 134–149.
- , and (1999) Linking asymmetric division to cell fate: teaching an old microbe new tricks. Genes Dev 13: 377–381.
- , and (1992) Crisscross regulation of cell-type-specific gene expression during development in Bacillus subtilis. Nature 355: 601–604.
- , , and (2001) Growth-rate regulation of the Bacillus subtilis accBC operon encoding subunits of the acetyl-CoA carboxylase, the first enzyme of fatty acid synthesis. Arch Microbiol 175: 234–237.
- , , and (2010) Transcriptional and functional characterization of the gene encoding acyl carrier protein in Bacillus subtilis. Microbiology 156: 484–495.
- , , , and (2006) Lipid domains in bacterial membranes. Mol Microbiol 61: 1110–1117.
- , , , and (2010) Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis. Mol Microbiol 76: 956–970.Direct Link:
- , and (2005) Role of membrane lipids in bacterial division-site selection. Curr Opin Microbiol 8: 135–142.
- (1970) Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol 49: 415–432.
- , , , , , , and (2003) The Spo0A regulon of Bacillus subtilis. Mol Microbiol 50: 1683–1701.
- , , , , and (2010) A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis. Genes Dev 24: 411–422.
- , and (1988) An amphiphilic polysaccharide from an adhesive Rhodococcus strain. FEMS Microbiol Lett 49: 389–392.
- , , , and (1992) Structural studies of an emulsion-stabilizing exopolysaccharide produced by an adhesive, hydrophobic Rhodococcus strain. J Gen Microbiol 138: 2531–2537.
- , , , , and (2005) Phosphatydylethanolamine domains and localization of phospholipids synthases in Bacillus subtilis membranes. J Bacteriol 187: 2163–2174.
- , and (2005) Protein localization: reach out and touch the forespore. Curr Biol 15: 165–167.
- , and (2009) Negative membrane curvature as a cue for subcellular localization of a bacterial protein. Proc Natl Acad Sci USA 106: 13541–13545.
- , , , , and (2003) Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in C. elegans embryonic polarity. BMC Dev Biol 3: 1–15.
- , , , , , and (2008) The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of Bacillus subtilis. Mol Microbiol 69: 1104–1120.
- , , , and (2010) Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. Proc Natl Acad Sci USA 107: 2230–2234.
- , , and (2002) Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture. Proc Natl Acad Sci USA 99: 8701–8706.
- , and (1997) Organelle structure, function, and inheritance in yeast: a role for fatty acid synthesis? Cell 88: 431–434.
- , , , , and (1998) De novo fatty acid synthesis is required for establishment of cell type specific gene transcription during sporulation in Bacillus subtilis. Mol Microbiol 29: 1215–1224.Direct Link:
- , and (2011) Extracellular signaling and multicellularity in Bacillus subtilis. Curr Opin Microbiol 14: 741–747.
- , and (1972) The fluid mosaic model of the structure of cell membranes. Science 175: 720–732.
- , and (1996) Molecular genetics of sporulation in Bacillus subtilis. Annu Rev Genet 30: 297–341.
- , , and (1998) Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression. Cell 52: 697–704.
- , , , , , , et al. (2009) Glycolipids are involved in biofilm accumulation and prolongued bacteraemia in Enterococcus faecalis. Mol Microbiol 71: 1055–1069.
- , , and (1998) A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144: 3097–3104.
- , , and (2003) Phosphatydylethanolamine and phosphatydylglycerol are segregated into different domains in bacterial membrane. A study with pyren-labelled phospholipids. Mol Microbiol 49: 1067–1079.
- , , , and (2012) Synergistic effects of probiotic Leuconostoc mesenteroides and Bacillus subtilis in malted ragi food for antagonistic activity against V. cholerae and other beneficial properties. J Food Sci Technol doi: 10.1007/s13197-012-0834-5
- (1996) PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12: 259–265.
- , and (2008) Membrane lipid homeostasis in bacteria. Nat Rev Microbiol 6: 222–233.

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