These authors contributed equally to this work.
ComE/ComE∼P interplay dictates activation or extinction status of pneumococcal X-state (competence)
Article first published online: 10 DEC 2012
© 2012 Blackwell Publishing Ltd
Volume 87, Issue 2, pages 394–411, January 2013
How to Cite
Martin, B., Soulet, A.-L., Mirouze, N., Prudhomme, M., Mortier-Barrière, I., Granadel, C., Noirot-Gros, M.-F., Noirot, P., Polard, P. and Claverys, J.-P. (2013), ComE/ComE∼P interplay dictates activation or extinction status of pneumococcal X-state (competence). Molecular Microbiology, 87: 394–411. doi: 10.1111/mmi.12104
- Issue published online: 14 JAN 2013
- Article first published online: 10 DEC 2012
- Manuscript Accepted: 14 NOV 2012
- Ministère Délégué à la Recherche et aux Nouvelles Technologies. Grant Number: RB/CD/2003/09/001
- Agence Nationale de la Recherche. Grant Number: Projet 2010 Blanc SVSE 3: PneumocoX
- Programme Microbiologie 2003-2004
- Association pour la Recherche sur le Cancer
Since 1996, induction of competence for genetic transformation of Streptococcus pneumoniae is known to be controlled by the ComD/ComE two-component regulatory system. The mechanism of induction is generally described as involving ComD autophosphorylation, transphosphorylation of ComE and transcriptional activation by ComE∼P of the early competence (com) genes, including comX which encodes the competence-specific σX. However, none of these features has been experimentally established. Here we document the autokinase activity of ComD proteins in vitro, and provide an estimate of the stoichiometry of ComD and ComE in vivo. We report that a phosphorylmimetic mutant, ComED58E, constructed because of the failure to detect transphosphorylation of purified ComE in vitro, displays full spontaneous competence in ΔcomD cells, an that in vitro ComED58E exhibits significantly improved binding affinity for PcomCDE. We also provide evidence for a differential transcriptional activation and repression of PcomCDE and PcomX. Altogether, these data support the model of ComE∼P-dependent activation of transcription. Finally, we establish that ComE antagonizes expression of the early com genes and propose that the rapid deceleration of transcription from PcomCDE observed even in cells lacking σX is due to the progressive accumulation of ComE, which outcompetes ComE∼P.