Concise description: The architecture of Gram-positive peptidoglycan is still not established, despite decades of research, partially due to a lack of methods to directly visualize it because of it's intermediate size. Here we use electron cryo-tomography to image Gram-positive peptidoglycan in 3D, and combine this with molecular dynamics simulations to bridge scales. Multiple lines of evidence from our work, together with multiple lines of evidence by previous researchers, strongly support the model in which glycan strands run circumferentially around the long axis of the cell.
Architecture and assembly of the Gram-positive cell wall
Version of Record online: 22 APR 2013
© 2013 John Wiley & Sons Ltd
Volume 88, Issue 4, pages 664–672, May 2013
How to Cite
Beeby, M., Gumbart, J. C., Roux, B. and Jensen, G. J. (2013), Architecture and assembly of the Gram-positive cell wall. Molecular Microbiology, 88: 664–672. doi: 10.1111/mmi.12203
- Issue online: 13 MAY 2013
- Version of Record online: 22 APR 2013
- Manuscript Accepted: 4 MAR 2013
- National Institute of Health. Grant Number: R01 GM062342
- Argonne Director's Postdoctoral Fellowship
- Howard Hughes Medical Institute
- Gordon and Betty Moore Center for Integrative Study of Cell Regulation at Caltech
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