These authors contributed equally to this work.
An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis
Article first published online: 13 DEC 2013
© 2013 John Wiley & Sons Ltd
Volume 91, Issue 2, pages 348–362, January 2014
How to Cite
Rueff, A.-S., Chastanet, A., Domínguez-Escobar, J., Yao, Z., Yates, J., Prejean, M.-V., Delumeau, O., Noirot, P., Wedlich-Söldner, R., Filipe, S. R. and Carballido-López, R. (2014), An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis. Molecular Microbiology, 91: 348–362. doi: 10.1111/mmi.12467
The authors have no conflict of interest to declare.
- Issue published online: 10 JAN 2014
- Article first published online: 13 DEC 2013
- Accepted manuscript online: 22 NOV 2013 03:41AM EST
- Manuscript Accepted: 18 NOV 2013
- ‘Fundação para a Ciência e Tecnologia’. Grant Numbers: PTDC/BIA-MIC/100747/2008, PEst-OE/EQB/LA0004/2011
- Human Frontier Science Program Organization. Grant Number: HFSP-RGY0067/2009-C
- Max-Planck society
- French National Research Agency. Grant Number: ANR-08-JCJC-0024-01
- Marie Curie IRG. Grant Number: 249018
- MICA department (INRA)
MreB proteins play a major role during morphogenesis of rod-shaped bacteria by organizing biosynthesis of the peptidoglycan cell wall. However, the mechanisms underlying this process are not well understood. In Bacillus subtilis, membrane-associated MreB polymers have been shown to be associated to elongation-specific complexes containing transmembrane morphogenetic factors and extracellular cell wall assembly proteins. We have now found that an early intracellular step of cell wall synthesis is also associated to MreB. We show that the previously uncharacterized protein YkuR (renamed DapI) is required for synthesis of meso-diaminopimelate (m-DAP), an essential constituent of the peptidoglycan precursor, and that it physically interacts with MreB. Highly inclined laminated optical sheet microscopy revealed that YkuR forms uniformly distributed foci that exhibit fast motion in the cytoplasm, and are not detected in cells lacking MreB. We propose a model in which soluble MreB organizes intracellular steps of peptidoglycan synthesis in the cytoplasm to feed the membrane-associated cell wall synthesizing machineries.