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mpp12000-sup-0001-si.tif467K

Fig. S1 (A) qRT-PCR analyzing bZIP60, BiP and SKP1 transcript abundance. The plots show the relative abundance of transcripts due to each TMV, PVX, and TMV plus PVX infection. Clearly transcript abundance is higher during PVX infection. Furthermore, TMV has a dominant suppressive effect on the ability of PVX to induce host gene expression. Furthermore, we fused the PVX replicase, TGBp1, and CP to the CaMV35S promoter and delivered these plasmids to plants using agro-infiltration. qRT-PCR shows host gene expression is not induced by these factors. Immunoblots demonstrating the relative abundance of TGBp1 (B), PVX CP (D), and commassie stained gel detecting TGBp2 (C) when expressed from either PVX or TMV vector in infected N. benthamiana leaves. Since we lack antisera to TGBp2, commassie staining was used to identify a band that likely corresponds to TGBp2 in infected leaf extracts. Commassie stained immunoblot membranes in panels B and D show similar levels of protein in each lane.

mpp12000-sup-0002-si.tif511K

Fig. S2 Immunoblot analysis detecting PVX TGBp3-His protein using antisera detecting the His tag. (A) Leaf extracts from mock inoculated (M; lane 1), PVX-TGBp3His (lanes 2–5), TMV-p3His (lanes 6–9), and agrobacterium (lane 11) expressing TGBp3His from CaMV 35S promoter (lanes 12–13). Leaf extracts were taken from virus-inoculated (Inoc) and systemically (Syst) infected tissues. TMV-p3His showed higher levels of protein accumulation than PVX-TGBp3His and required 20 s exposure to film to see abundance of protein in the top panel. To visualize TGBp3His expressed from PVX, we exposed the film for 1 min and this is shown in the middle panel. TGBp3His expression from Agrobacterium was comparable to TMV-p3His as seen after 20 s exposure to film. Below is a commassie stained PVDF membrane photographed after electrophoretic transfer to show protein levels in each lane were comparable. (B) Immunoblot detects TMV coat protein. TMV-p3 and TMV-p3His were detected in systemic tissues and produced comparable levels of TMV coat protein indicating that the His tag is not deleterious to virus infection.

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