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mpp12002-sup-0001-si.tif2502K

Fig. S1 Expression of enhanced green fluorescent protein (EGFP) fused to the wild-type and Chinese wheat mosaic virus (CWMV) cysteine-rich protein (CRP) mutants in epidermal cells. Leaves of Nicotiana benthamiana plants were infiltrated with Agrobacterium harbouring a binary vector containing the constructs indicated above the images. EGFP fluorescence was observed using confocal laser scanning microscopy (CLSM) at 3 days after inoculation (dai). Bars, 30 μm.

mpp12002-sup-0002-si.tif4442K

Fig. S2 Accumulation of enhanced green fluorescent protein (EGFP) fused with Chinese wheat mosaic virus (CWMV) cysteine-rich protein (CRP) mutants in the perinuclear region. Leaves of Nicotiana benthamiana plants were infiltrated with Agrobacterium harbouring a binary vector containing the constructs indicated above the images. EGFP fluorescence and 4′,6-diamidino-2-phenylindole (DAPI) staining in epidermal cells were observed using confocal laser scanning microscopy (CLSM) at 3 days after inoculation (dai). Bars, 10 μm.

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