Fig. S1 Callose deposition in untreated (Ctr) and hexanoic acid-treated (Hx) plants on infection with Pseudomonas syringae. Leaves of tomato plants were sampled, stained with calcofluor/aniline blue and analysed by epifluorescence microscopy. Quantification was performed by determining the number of yellow pixels/million corresponding to pathogen-induced callose on digital photographs of infected leaves. Data show average valuesn ± standard error (SE) (n = 10). Different letters represent statistically significant differences (P < 0.05; least-significant difference test). Higher magnification views are shown of callose deposition in tomato leaves infected with P. syringae visualized with bright light and UV.


Fig. S2 Expression analyses of marker genes PR5, Asr1 and AccOx for salicylic acid (SA), abscisic acid (ABA) and ethylene (ET) signalling pathways, respectively, in priming by hexanoic acid against Pseudomonas syringae. Tomato plants were grown, treated and inoculated as described in Experimental procedures. Genes were analysed in cDNA from untreated and uninoculated plants (Ctr), untreated and inoculated plants (Ctr inf), treated plants (Hx), and treated and inoculated plants (Hx inf) at different time points. Results show average values of three independent experiments with similar results ± standard error (SE) (n = 3).

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