Hexanoic acid is a resistance inducer that protects tomato plants against Pseudomonas syringae by priming the jasmonic acid and salicylic acid pathways
Article first published online: 23 DEC 2012
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Molecular Plant Pathology
Volume 14, Issue 4, pages 342–355, May 2013
How to Cite
Scalschi, L., Vicedo, B., Camañes, G., Fernandez-Crespo, E., Lapeña, L., González-Bosch, C. and García-Agustín, P. (2013), Hexanoic acid is a resistance inducer that protects tomato plants against Pseudomonas syringae by priming the jasmonic acid and salicylic acid pathways. Molecular Plant Pathology, 14: 342–355. doi: 10.1111/mpp.12010
- Issue published online: 2 APR 2013
- Article first published online: 23 DEC 2012
- National R&D Plan. Grant Numbers: AGL2010-22300-C03-01, AGL2010-22300-C03-02
- Ministerio de Educación. Grant Number: AP2008-01064
Fig. S1 Callose deposition in untreated (Ctr) and hexanoic acid-treated (Hx) plants on infection with Pseudomonas syringae. Leaves of tomato plants were sampled, stained with calcofluor/aniline blue and analysed by epifluorescence microscopy. Quantification was performed by determining the number of yellow pixels/million corresponding to pathogen-induced callose on digital photographs of infected leaves. Data show average valuesn ± standard error (SE) (n = 10). Different letters represent statistically significant differences (P < 0.05; least-significant difference test). Higher magnification views are shown of callose deposition in tomato leaves infected with P. syringae visualized with bright light and UV.
Fig. S2 Expression analyses of marker genes PR5, Asr1 and AccOx for salicylic acid (SA), abscisic acid (ABA) and ethylene (ET) signalling pathways, respectively, in priming by hexanoic acid against Pseudomonas syringae. Tomato plants were grown, treated and inoculated as described in Experimental procedures. Genes were analysed in cDNA from untreated and uninoculated plants (Ctr), untreated and inoculated plants (Ctr inf), treated plants (Hx), and treated and inoculated plants (Hx inf) at different time points. Results show average values of three independent experiments with similar results ± standard error (SE) (n = 3).
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