Small-molecule inhibitors suppress the expression of both type III secretion and amylovoran biosynthesis genes in Erwinia amylovora
Article first published online: 5 AUG 2013
© 2013 BSPP AND JOHN WILEY & SONS LTD
Molecular Plant Pathology
Volume 15, Issue 1, pages 44–57, January 2014
How to Cite
Yang, F., Korban, S. S., Pusey, P. L., Elofsson, M., Sundin, G. W. and Zhao, Y. (2014), Small-molecule inhibitors suppress the expression of both type III secretion and amylovoran biosynthesis genes in Erwinia amylovora. Molecular Plant Pathology, 15: 44–57. doi: 10.1111/mpp.12064
- Issue published online: 5 DEC 2013
- Article first published online: 5 AUG 2013
- Accepted manuscript online: 19 JUL 2013 05:43AM EST
- USDA National Institute of Food and Agriculture (YFZ). Grant Number: 2010-5110-20497
- USDA-SCRI. Grant Number: AG 2009-1181-06023(SSK)
Fig. S1 Promoter activities of type III secretion system (T3SS) genes of Erwinia amylovora in M9 and hrp (hypersensitive response and pathogenicity)-inducing minimal medium (HMM) with different concentrations of carbon sources. (A) Promoter activities of hrpN, dspE, hrpL and hrpA in E. amylovora strain Ea273 in M9 and HMM containing 10 and 20 mm of galactose at 18 °C for 18 h. (B) Time course of promoter activities of hrpN, dspE, hrpL and hrpA in E. amylovora strain Ea273 in HMM containing 20 mm galactose at 18 °C.
Fig. S2 Effect of different concentrations of small-molecule inhibitors 3 and 9 on promoter activities of hrpN, dspE, hrpL and hrpA genes. Erwinia amylovora strain Ea273 was grown in hrp (hypersensitive response and pathogenicity)-inducing minimal medium (HMM) at 18 °C for 18 h. The highest volume of dimethylsulphoxide (DMSO) added (equivalent to 100 μm of chemicals) is shown as the control.
Fig. S3 (A) Effect of small molecules 3 and 9 on bacterial growth in hrp (hypersensitive response and pathogenicity)-inducing minimal medium (HMM) containing 20 mm galactose at 18 °C. (B) Effect of small molecule 3 on bacterial growth on flowers. Flowers were homogenized at 0, 2, 4 and 6 days post-inoculation. The population was measured by plating serial dilutions and calculating colony-forming units (CFU).
Fig. S4 Small-molecule inhibitors delayed the hypersensitive response (HR) in tobacco. Tobacco leaf was infiltrated with Erwinia amylovora Ea1189 (OD600 = 0.15) in the presence of compounds 8 (left) and 11 (right) at the different concentrations indicated. The same volume of dimethylsulphoxide (DMSO) was added as a control as 100 μm of compounds. Photographs were taken 24 h post infiltration.
Fig. S5 Structures of the small-molecule inhibitors used in this study. Not shown are compounds 6 (see Felise et al., 2008; compound #1, TTS29), 7, 8, 15 and 16 (see Pan et al., 2009; compounds #2, 1, 3 and 3-diproprionate, respectively).
Table S1 Differentially expressed genes in all comparisons.
Table S2 Promoter cloning and quantitative real-time polymerase chain reaction (qRT-PCR) primers used in this study.
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