This work was submitted as partial requirement for the DSc. degree of Dr Costa at the Universidade Federal do Rio Grande do Sul.
Genetic variability in Microsporum canis isolated from cats, dogs and humans in Brazil†
Article first published online: 2 APR 2013
© 2013 Blackwell Verlag GmbH
Volume 56, Issue 5, pages 582–588, September 2013
How to Cite
da Costa, F. V. A., Farias, M. R., Bier, D., de Andrade, C. P., de Castro, L. A., da Silva, S. C. and Ferreiro, L. (2013), Genetic variability in Microsporum canis isolated from cats, dogs and humans in Brazil. Mycoses, 56: 582–588. doi: 10.1111/myc.12078
- Issue published online: 26 JUL 2013
- Article first published online: 2 APR 2013
- Manuscript Accepted: 6 MAR 2013
- Manuscript Revised: 13 JAN 2013
- Manuscript Received: 11 OCT 2012
- CNPq. Grant Number: 140591/2007-9
- microsatellite repeats;
Dermatophytosis caused by Microsporum canis is a heterogeneous disease with variable clinical manifestations. M. canis is a zoophilic dermatophyte and the most frequent fungi isolated from dogs, cats and children in Brazil. The aim of this study was to investigate the genetic variability of M. canis isolates from different animal species using two microsatellite markers, namely, McGT(13) and McGT(17), and to correlate the results with the clinical and epidemiological patient data in Brazil. The study included a global set of 102 M. canis strains, including 37 symptomatic cats, 35 asymptomatic cats, 19 human patients with tinea, 9 asymptomatic dogs and 2 symptomatic dogs. A total of 14 genotypes were identified, and 6 large populations were distinguished. There was no correlation between these multilocus genotypes and the clinical and epidemiological data, including the source, symptomatology, clinical picture, breed, age, sex, living conditions and geographic location. These results demonstrate that the use of microsatellite polymorphisms is a reliable method for the differentiation of M. canis strains. However, we were unable to demonstrate a shared clinical and epidemiological pattern among the same genotype samples.