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Cryptococcus gattii in urban trees from cities in North-eastern Argentina

Authors

  • Mariana Mazza,

    Corresponding author
    1. Mycology Department, INEI ‘Dr. Carlos G. Malbrán’- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
    • Correspondence: Mariana Mazza, Mycology Department, INEI ‘Dr. Carlos G. Malbrán', ANLIS. Av. Vélez Sarsfield 563, C1282AFF, Ciudad Autónoma de Buenos Aires, Argentina.

      Tel.: +54 11 4302-5066 int. *38. Fax: +54 11 4302-5066 int. *30.

      E-mail: pnccm@anlis.gov.ar

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  • Nicolás Refojo,

    1. Mycology Department, INEI ‘Dr. Carlos G. Malbrán’- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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  • María Eugenia Bosco-Borgeat,

    1. Mycology Department, INEI ‘Dr. Carlos G. Malbrán’- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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  • Constanza Giselle Taverna,

    1. Mycology Department, INEI ‘Dr. Carlos G. Malbrán’- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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  • Alicia Cristina Trovero,

    1. Antigens and Antisera Laboratory, INPB - ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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  • Ariel Rogé,

    1. Antigens and Antisera Laboratory, INPB - ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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  • Graciela Davel

    1. Mycology Department, INEI ‘Dr. Carlos G. Malbrán’- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina
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Summary

In the city of Buenos Aires, Argentina, Cryptococcus gattii genotype AFLP4/VGI was found to be associated with decaying wood in hollows of different tree species. The aim of this study was to investigate the presence of C. gattii in the environment of riverside cities of the river Paraná, and to describe its serotypes and molecular types. Five hundred samples were collected in 50 parks by swabbing tree hollows. The samples were inoculated on caffeic acid agar supplemented with chloramphenicol, and incubated at 28 °C for 1 week with a daily observation. The isolates were identified by conventional methods. The serotype was determined by slide agglutination with specific antisera. Molecular typing was carried out by PCR-RFLP of the URA5 gene. Four isolates of C. gattii were recovered: Cryptococcus gattii serotype B, genotype AFLP4/VGI, isolated from Eucalyptus sp. in the city of Rosario and from Grevillea robusta in the city of La Paz; and C. gattii serotype C, genotype AFLP5/VGIII, isolated from two different Tipuana tipu trees in the city of Resistencia. Here, we report for the first time the isolation of C. gattii serotype C, genotype AFLP5/VGIII, from environmental samples in Argentina.

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