The cell survival kinase SGK1 and its targets FOXO3a and NDRG1 in aged human brain
Article first published online: 9 SEP 2013
© 2013 The Authors. Neuropathology and Applied Neurobiology © 2013 British Neuropathological Society
Neuropathology and Applied Neurobiology
Volume 39, Issue 6, pages 623–633, October 2013
How to Cite
Sahin, P., McCaig, C., Jeevahan, J., Murray, J. T. and Hainsworth, A. H. (2013), The cell survival kinase SGK1 and its targets FOXO3a and NDRG1 in aged human brain. Neuropathology and Applied Neurobiology, 39: 623–633. doi: 10.1111/nan.12023
- Issue published online: 9 SEP 2013
- Article first published online: 9 SEP 2013
- Accepted manuscript online: 30 JAN 2013 06:36AM EST
- Manuscript Accepted: 25 JAN 2013
- Manuscript Received: 6 JUL 2012
- Alzheimer's Research UK
- St Georges Hospital Charity
- The Neuroscience Research Foundation
- Action Medical Research
Figure S1. Test of SGK1 antibody specificity in aged human brain and human kidney. Anti-SGK1 antibody was immunodepleted using recombinant human SGK1, then used to probe for SGK1 in sections of human frontal cerebral cortex (A–B, E–F) and human renal cortex (Kidney; C–D, G–H). Characteristic cellular staining for SGK1 (brown), with nuclear localization, was seen in brain (A,E) and kidney (C,G) treated with undepleted antibody. Labelling was absent or much-reduced from adjacent sections of brain (B,F) and kidney (D,H) that had been treated with depleted antibody. Haematoxylin counterstain (blue). Scale bars: 200 μm (A–D), 20 μm (E–H).
Figure S2. Western data used for densitometry. Western blots of cortical tissue lysates from fourteen aged control subjects and fourteen severe AD patients (lanes labelled C, A respectively) probed either for phospho-NDRG1 (p3-NDRG1) or for total NDRG1.
Table S1. Neuropathological data on severe AD and aged control (AC) cases.
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