S.-Y.Y. and J.-U.C. equally contributes to this work.
α-Secretase cleaved amyloid precursor protein (APP) accumulates in cholinergic dystrophic neurites in normal, aged hippocampus
Article first published online: 28 OCT 2013
© 2013 British Neuropathological Society
Neuropathology and Applied Neurobiology
Volume 39, Issue 7, pages 800–816, December 2013
How to Cite
Yoon, S.-Y., Choi, J.-U., Cho, M.-H., Yang, K.-M., Ha, H., Chung, I. J., Cho, G. S. and Kim, D.-H. (2013), α-Secretase cleaved amyloid precursor protein (APP) accumulates in cholinergic dystrophic neurites in normal, aged hippocampus. Neuropathology and Applied Neurobiology, 39: 800–816. doi: 10.1111/nan.12032
- Issue published online: 28 OCT 2013
- Article first published online: 28 OCT 2013
- Accepted manuscript online: 18 FEB 2013 04:45AM EST
- Manuscript Accepted: 31 JAN 2013
- Manuscript Received: 9 FEB 2012
- Korean government. Grant Number: 2008-0062466
- Ministry of Education, Science and Technology. Grant Number: 2010-0002859
- Asan Institute for Life Sciences, Seoul, Korea. Grant Number: 10-067
Figure S1. DAB images of ageing associated dystrophic neurites. Coronal sections of the hippocampus from 15-month-old mice were immunostained with the labelled antibodies, which shows the same pattern as the fluorescence images. Arrowheads indicate clusters of dystrophic neurites. High-power images of clusters are shown next to the low-power images. Scale bar = 50 μm (Low power). Scale bar = 5 μm (High power).
Figure S2. (A) High-power confocal images of clusters of dystrophic neurites. Coronal sections of the hippocampus from 15-month-old mice were immunostained with the labelled antibodies and confocal images were taken. Scale bar = 5 μm. (B) Coronal sections of the hippocampus from 15-month-old mice were immunostained with the APP-NT antibody. Higher-power image of clusters in the square box is shown in the lower panel. Scale bar = 5 μm. (C) Transmission electron microscopy images of clusters of dystrophic neurites. High magnification image of a dystrophic neurite (arrowhead) filled with translucent or amorphous electrodense materials. Scale bar = 2 μm (upper panel). Scale bar = 500 nm (lower panel).
Figure S3. Accumulation of cathepsin-D and γ-tubulin in the dystrophic neurites of lactacystin-treated neurones. Treatment of primary cortical neurones with the proteasome inhibitor lactacystin (10 μM) for 24 h induced accumulation of cathepsin-D and γ-tubulin (arrowheads) in dystrophic neurites, indicating the accumulation of lysosomes and aggresomes. Scale bar = 20 μm.
Figure S4. Accumulation of α-secretase-cleaved APP peptides in the dystrophic neurites of lactacystin-treated neurones. (A) Immunofluorescence of various APP epitopes in control neurones. (B) After treating with 10 μM lactacystin for 24 h, dystrophic neurites that accumulated cathepsin-D were also immunopositive for 6E10 staining, which detects Aβ1–16 (arrowheads); however, immunostaining for the 4G8 antibody, which detects Aβ17–24, was less intense in dystrophic neurites and was not colocalized with cathepsin-D (arrows). APP-NT immunostaining was prominent in dystrophic neurites, but APP-CT695 immunostaining was light and did not colocalize with APP-NT (arrowheads), indicating the predominant accumulation of α-secretase-cleaved APP peptides in the dystrophic neurites of lactacystin-treated neurones. Scale bar = 20 μm.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.