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nan12047-sup-0001-fs1.pptx3467K

Figure S1. The distribution of PrP-positive microspheres. (A) Haematoxylin and eosin stain. (B, C, D) Immunohistochemistry for prion protein (clone 3F4). (E) Schema showing distribution of amyloid microspheres. Numerous microspheres are distributed at the layer of pre-α neurone (asterisks) in the transentorhinal cortex (A, B), in the molecular layer of the cerebellar cortex (C) and in the central grey matter of the midbrain (D). (E) Microspheres are widely distributed in the cerebral neocortices, entorhinal cortex and transentorhinal cortex, but focally detected in the molecular layer of the cerebellum.

nan12047-sup-0002-fs2.pptx239K

Figure S2. Western blotting for PrP in brain homogenates prepared in lysis buffer with 1% sodium dodecyl sulphate. (A) In the sample without PK treatment, Western blotting using 3F4 antibody reveals nonglycoform, monoglycoform, diglycoform PrP bands. In addition, a PrP fragment around 15 kDa is also noted (arrow). (B) In the sample without PK treatment, the PrP fragment around 15 kDa is not detected by Western blotting using the 8H4 antibody. (C) In the fractionated sample (f3) with PK treatment, the fragment around 13 kDa is not detected by Western blotting using the 8H4 antibody. * (asterisks): loading sample.

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