Figure S1. Astrocytic and microglial activation. (a) Stratum lacunosum-moleculare (SL) showing moderate astrocytosis and mild vacuolation. Bar = 200 μm. Immunohistochemistry for GFAP. (b) Stratum lacunosum-moleculare showing mild microglial activation and mild vacuolation. Bar = 100 μm. Immunohistochemistry for coronin. (c) Severe astrocytosis in the superior colliculus associated with marked vacuolation. Bar = 200 μm. Immunohistochemistry for GFAP. (d) Severe microgliosis associated with marked vacuolation in the midbrain tectum. Bar = 100 μm. Immunohistochemistry for coronin. (e) Marked astrocytosis is present around several sub-ependymal plaques (arrowheads). Bar = 100 μm. Immunohistochemistry for GFAP. (f) Small numbers of activated microglia are present in the neuropil adjacent to amyloid plaques (arrowheads) in the periventricular glial limitans. Bar = 100 μm. Immunohistochemistry for coronin.


Figure S2. Tau immunolabelling: cerebral cortex showing moderate vacuolation and punctuate tau immunolabelled structures. Bar = 50 μm. Immunohistochemistry for tau.


Figure S3. Area postrema. (a) A large areas of neuropil surrounding a blood vessel shows loss of normal architecture, increased extracellular space and a dystrophic neurite (d). Bar = 3 μm. Uranyl acetate/lead citrate. (b) Detail of an area similar to the above, showing loss of normal cellular processes and replacement by poorly defined fibrillar (arrowheads) PrPSSLOW amyloid fibrils. Bar = 0.5 μm. Immunogold for PrPSSLOW. (c) Accumulation of PrPSSLOW in association with convoluted and microfolded processes, and poorly defined fibrils (arrowheads) surrounding a large astrocytic process (A). Bar = 1 μm. Immunogold for PrPSSLOW.


Figure S4. Enlarged mitochondria: enlarged mitochondria with abnormal christae were mostly located in glial cell cytoplasm. A normal mitochondrion is present at M. Bar = −0.5 μm. Uranyl acetate/lead citrate.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.