Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis
Version of Record online: 21 JAN 2014
© 2013 British Neuropathological Society
Neuropathology and Applied Neurobiology
Volume 40, Issue 2, pages 191–204, February 2014
How to Cite
Crèvecœur, J., Kaminski, R. M., Rogister, B., Foerch, P., Vandenplas, C., Neveux, M., Mazzuferi, M., Kroonen, J., Poulet, C., Martin, D., Sadzot, B., Rikir, E., Klitgaard, H., Moonen, G. and Deprez, M. (2014), Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis. Neuropathology and Applied Neurobiology, 40: 191–204. doi: 10.1111/nan.12054
- Issue online: 21 JAN 2014
- Version of Record online: 21 JAN 2014
- Accepted manuscript online: 26 APR 2013 05:37AM EST
- Manuscript Accepted: 17 APR 2013
- Manuscript Received: 11 JAN 2013
- SPW (Service Public de Wallonie)
- Neurocom project. Grant Number: n°716747
- Neuredge project. Grant Number: n°816859
Supplementary material and methods.
Figure S1. SV2C positive controls. (a) SV2C immunoreactivity (IR) in human globus pallidus. Characteristic ‘wooly fibres’ are labelled (scale bar: 200 μm). (b) Western blot analysis: 1 = olfactive bulb of wild-type mouse, 2 = striatum of wild-type mouse, 3 = control human hippocampus, 4 = control human striatum, 5 = control human globus pallidus. 1 and 2 are positive controls.
Figure S2. Scores of immunoreactivity (IR) for SV2C, dynorphin and ZnT3 in the inner molecular layer (IML) of the dentate gyrus. Intensity of IR for dynorphin, ZnT3 and SV2C in the IML was expressed as semi-quantitative score: 0 when the IR pattern was similar to controls; and 1, 2 or 3 for respectively mild, moderate or severe increase of IR in the IML. Scale bar = 200 μm.
Table S1. mRNA values for SV2A, SV2B and SV2C determined by bDNA assay in controls and temporal lobe epilepsy (TLE) patients. Experiments have been carried out in triplicate and the mean value of the three experiments is displayed.
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