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Cellular sources of cyclooxygenase-1 and -2 up-regulation in the spinal dorsal horn after spinal nerve ligation
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Neuropathology and Applied Neurobiology
- Accepted manuscript online: 31 JUL 2013 02:46AM EST
- Manuscript Accepted: 29 JUL 2013
- Cited By
- Neuropathic pain;
- spinal cord;
Recent studies suggested that the development of neuropathic pain associated with neural injury may be partly due to up-regulation of cyclooxygenase (COX) in the CNS. However, the cellular sources of COX-1 and COX-2 up-regulation following nerve injury are unclear.
We investigated the spinal cellular sources of COX-1 and COX-2 in association with allodynia following L5 spinal nerve ligation (SNL).
Post-SNL pain-related behaviour was shown by increased sensitivity to mechanical stimulation. There was a significant increase in both COX-1 and COX-2 immunoreactivity (p<0.01) on the ipsilateral side of spinal dorsal horn. Double immunofluorescence labeling demonstrated that COX-1 immunoreactive cells co-localized chiefly with dorsal horn neuronal nuclei and microglia, whereas COX-2 was expressed in neuronal cytoplasm.
These findings demonstrate that while spinal dorsal horn neurons are important source of COX-1 and COX-2 after nerve injury, microglia also contribute to the pathogenesis of neuropathic pain, partly by producing additional COX-1.