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Figure S1. REST immunoreactivity in layers II, III and V of the frontal cortex of a control subject. DAB, 100×. Scale bar: 50 μm.

Figure S2. REST in frontal cortex of a control subject. An intense nuclear REST immunoreactivity is observed in neurones with nuclear hyperchromatosis (arrowheads). Arrows point at glial cells with intense REST staining. DAB, 400×. Scale bar: 50 μm.

Figure S3. REST immunoreactivity in the pyramidal layer of the hippocampus of a control subject. DAB, 200×. Scale bar: 50 μm.

Figure S4. REST immunoreactivity in the cerebellum of a control subject. DAB, 100×. Scale bar: 50 μm.

Figure S5. REST staining on serial sections of control caudate nucleus, cortex, hippocampus and cerebellum in the absence and in the presence of the REST blocking peptide. DAB, 400×. Scale bars: 50 μm.

Figure S6. REST staining on serial sections of HD caudate nucleus, cortex, white matter and hippocampus in the absence and in the presence of the REST blocking peptide. DAB, 400×. Scale bars: 50 μm.

Figure S7. HTT expression in the control brain. (A) Frontal cortex: the arrowhead indicates a neurone showing HTT immunoreactivity in the cytoplasm. DAB, 200×. (B) Frontal cortex white matter: arrows indicate glial cells positive for HTT, DAB 200×. (C) Caudate nucleus: arrowheads indicate neurones with cytoplasmic immunostaining for HTT. DAB, 400×. (D) Granular gyrus of hippocampus: HTT immunoreactivity in granular gyrus of hippocampus. DAB, 400×. (E) Pyramidal layer of the hippocampus: HTT immunoreactivity in neuronal cytoplasms. DAB 200×. (F) Cerebellum: HTT immunoreactivity in Purkinje cells (arrowheads). DAB, 400×. Scale bars: 50 μm.

Figure S8. HTT expression in the HD brain (grade III and IV). (A) Frontal cortex: low HTT immunoreactivity in neurones (arrowheads). HTT immunoreactivity in glial cells (arrows). DAB, 200×. (B) Caudate nucleus: low level of HTT expression in neuronal cytoplasm (arrowheads) and positive expression in glial cells (arrows). DAB, 400×. (C) Caudate nucleus: HTT is expressed in glial cells (arrows). DAB, 1000×. (D) Dentate gyrus of the hippocampus: low expression of HTT in the neuronal cytoplasm with a positive reinforcement on the nuclear membrane (arrowheads). DAB, 400×. (E) Hippocampus, pyramidal cells of Ammon's Horn (arrowheads): low expression of HTT. DAB, 400×. (F) Hippocampus white matter: HTT is expressed in many glial nuclei (arrows). DAB, 400×. Scale bars: 50 μm.

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