The natural history of cerebellar degeneration of Niemann-Pick C mice monitored in vitro
Version of Record online: 17 NOV 2014
© 2014 British Neuropathological Society
Neuropathology and Applied Neurobiology
Volume 40, Issue 7, pages 933–945, December 2014
How to Cite
Marschalek, N., Albert, F., Meske, V. and Ohm, T. G. (2014), The natural history of cerebellar degeneration of Niemann-Pick C mice monitored in vitro. Neuropathology and Applied Neurobiology, 40: 933–945. doi: 10.1111/nan.12154
- Issue online: 17 NOV 2014
- Version of Record online: 17 NOV 2014
- Accepted manuscript online: 30 MAY 2014 01:55AM EST
- Manuscript Accepted: 15 APR 2014
- Manuscript Received: 8 NOV 2013
Figure S1. Development of cerebellar slices during the culture period. After preparation (A) slices are thick and folia are difficult to identify. After 5 days in vitro (B) the initial reorganization process has flattened the slice. This process is finished after 10 days in vitro (C). Thereafter the morphology of the cultures is maintained well, after 21 (D) as well as after 42 days (E, F) in vitro.
Figure S2. Haematoxylin and eosin (H.E.) stainings of wild-type (A, B) and NPC −/− mice (C, D) after 42 days in vitro. The typical architecture of the cerebellum (granule cell layer, Purkinje cell layer, molecular cell layer) is visible. Purkinje cells are only recognizable in wild-type cultures (arrows). In NPC −/− slices Purkinje cells are not identifiable. Another hallmark of NPC disease is visible in picture (C). Large, lipid-loaden macrophages are formed during the progression of the disease.
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