Figure S1. Development of cerebellar slices during the culture period. After preparation (A) slices are thick and folia are difficult to identify. After 5 days in vitro (B) the initial reorganization process has flattened the slice. This process is finished after 10 days in vitro (C). Thereafter the morphology of the cultures is maintained well, after 21 (D) as well as after 42 days (E, F) in vitro.

Figure S2. Haematoxylin and eosin (H.E.) stainings of wild-type (A, B) and NPC −/− mice (C, D) after 42 days in vitro. The typical architecture of the cerebellum (granule cell layer, Purkinje cell layer, molecular cell layer) is visible. Purkinje cells are only recognizable in wild-type cultures (arrows). In NPC −/− slices Purkinje cells are not identifiable. Another hallmark of NPC disease is visible in picture (C). Large, lipid-loaden macrophages are formed during the progression of the disease.

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