nmo12083-sup-0001-FigS1.tifimage/tif5106KFigure S1. (A) Micrograph showing the distinctive mucosal folds (arrowheads) in the proximal colon of an Ednrb het animal. (B) Nikon Elements was used to separate representative Z projections of myenteric (green) and submucosal (red) plexus from the Ednrb het proximal colon. Scale bar =  2 mm (A), 100 μm (B).
nmo12083-sup-0002-FigS2.tifimage/tif4671KFigure S2. Representative Z projection micrographs of Hu and Substance P expression and proportion of Substance P expressing myenteric neurons in colchicine treated Ednrb het and null DMC. The density of neurons is reduced in the Ednrb nulls compared with hets (upper panel) although the relative percentage of Substance P neurons is similar (lower panel). Scale bar = 100 μm.
nmo12083-sup-0003-FigS3.tifimage/tif1618KFigure S3. Expression of nNOS in sacral-derived cells in the DMC of the Ednrb null colon. A small, thin ganglion found at the edge of the transition zone superficial to the longitudinal muscle, probably derived from the sacral neural crest, contains a high percentage of nNOS expressing Hu+ neurons and fibers (yellow). Scale bar = 50 μm.
nmo12083-sup-0004-TableS1.docWord document39KTable S1. Table of antibodies used in this study.

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