Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods
Article first published online: 11 MAR 2013
© 2013 John Wiley & Sons Ltd
Neurogastroenterology & Motility
Volume 25, Issue 6, pages 521–528, June 2013
How to Cite
Nishino, R., Mikami, K., Takahashi, H., Tomonaga, S., Furuse, M., Hiramoto, T., Aiba, Y., Koga, Y. and Sudo, N. (2013), Commensal microbiota modulate murine behaviors in a strictly contamination-free environment confirmed by culture-based methods. Neurogastroenterology & Motility, 25: 521–528. doi: 10.1111/nmo.12110
- Issue published online: 14 MAY 2013
- Article first published online: 11 MAR 2013
- Manuscript Accepted: 5 FEB 2013
- Manuscript Received: 25 JUN 2012
- Research Fund of Tokai University School of Medicine
- General Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology in Japan. Grant Numbers: 19390192, 22659144, 24659350
- Yakult Bioscience Research Foundation
There is increasing evidence suggesting the existence of an interaction between commensal microbiota, the gut and the brain. The aim of this study was to examine the influence of commensal microbiota on the host behaviors in a contamination-free environment, which was verified by culture-based methods.
Open-field and marble-burying tests were used to analyze anxiety-like behaviors and locomotor activity in gnotobiotic BALB/c mice with a common genetic background in a sterile isolator. The monoamine levels in several regions of the brain were measured in germfree (GF) mice and commensal fecal microbiota-associated mice (EX-GF).
A 24-h exposure to the environment outside the sterile isolators rendered GF mice less anxious than those not contaminated, while there was no change in the locomotion. EX-GF mice, the gnotobiotic mice with normal specific pathogen-free microbiota, were less anxious and active than GF mice using open-field and marble-burying tests. The norepinephrine, dopamine, and serotonin turnover rates were higher in the EX-GF mice than in the GF mice in most regions of the brain, suggesting that monoaminergic neurotransmission might increase in the EX-GF mice comparing the GF mice. Monoassociation with Brautia coccoides reduced the anxiety level, but it did not affect the locomotor activity. In contrast, colonization with Bifidobacterium infantis decreased the locomotor activity, while having little effect on the anxiety level.
Conclusions & Inferences
These results strongly support the current view that gut microorganisms modulate brain development and behavior.