Down-regulation of PvTRE1 enhances nodule biomass and bacteroid number in the common bean

Authors

  • Aarón Barraza,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Georgina Estrada-Navarrete,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Maria Elena Rodriguez-Alegria,

    1. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Agustin Lopez-Munguia,

    1. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Enrique Merino,

    1. Departamento de Microbiología Molecular, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Carmen Quinto,

    1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author
  • Federico Sanchez

    Corresponding author
    • Departamento de Biología Molecular de Plantas, Instituto de Biotecnología/Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México
    Search for more papers by this author

Author for correspondence:

Federico Sanchez

Tel: +52 777 329 1653

Email: federico@ibt.unam.mx

Summary

  • Legume–rhizobium interactions have been widely studied and characterized, and the disaccharide trehalose has been commonly detected during this symbiotic interaction. It has been proposed that trehalose content in nodules during this symbiotic interaction might be regulated by trehalase. In the present study, we assessed the role of trehalose accumulation by down-regulating trehalase in the nodules of common bean plants.
  • We performed gene expression analysis for trehalase (PvTRE1) during nodule development. PvTRE1 was knocked down by RNA interference (RNAi) in transgenic nodules of the common bean.
  • PvTRE1 expression in nodulated roots is mainly restricted to nodules. Down-regulation of PvTRE1 led to increased trehalose content (78%) and bacteroid number (almost one order of magnitude). In addition, nodule biomass, nitrogenase activity, and GOGAT transcript accumulation were significantly enhanced too.
  • The trehalose accumulation, triggered by PvTRE1 down-regulation, led to a positive impact on the legume–rhizobium symbiotic interaction. This could contribute to the agronomical enhancement of symbiotic nitrogen fixation.

Ancillary