Brassinosteroid production and signaling differentially control cell division and expansion in the leaf
Article first published online: 28 NOV 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 2, pages 490–502, January 2013
How to Cite
Zhiponova, M. K., Vanhoutte, I., Boudolf, V., Betti, C., Dhondt, S., Coppens, F., Mylle, E., Maes, S., González-García, M.-P., Caño-Delgado, A. I., Inzé, D., Beemster, G. T. S., De Veylder, L. and Russinova, E. (2013), Brassinosteroid production and signaling differentially control cell division and expansion in the leaf. New Phytologist, 197: 490–502. doi: 10.1111/nph.12036
- Issue published online: 18 DEC 2012
- Article first published online: 28 NOV 2012
- Manuscript Accepted: 7 OCT 2012
- Manuscript Received: 13 AUG 2012
- FP7 Marie-Curie Initial Training Network ‘BRAVISSIMO’. Grant Number: PITN-GA-2008-215118
- Belgian Science Policy (BELSPO)
- Agency for Innovation by Science and Technology
- Spanish Ministry of Science and Innovation. Grant Number: BIO2008/00505
- cell division;
- cpd ;
- Brassinosteroid (BR) hormones control plant growth through acting on both cell expansion and division. Here, we examined the role of BRs in leaf growth using the Arabidopsis BR-deficient mutant constitutive photomorphogenesis and dwarfism (cpd).
- We show that the reduced size of cpd leaf blades is a result of a decrease in cell size and number, as well as in venation length and complexity. Kinematic growth analysis and tissue-specific marker gene expression revealed that the leaf phenotype of cpd is associated with a prolonged cell division phase and delayed differentiation.
- cpd-leaf-rescue experiments and leaf growth analysis of BR biosynthesis and signaling gain-of-function mutants showed that BR production and BR receptor-dependent signaling differentially control the balance between cell division and expansion in the leaf.
- Investigation of cell cycle markers in leaves of cpd revealed the accumulation of mitotic proteins independent of transcription. This correlated with an increase in cyclin-dependent kinase activity, suggesting a role for BRs in control of mitosis.