An Arabidopsis reticulon and the atlastin homologue RHD3-like2 act together in shaping the tubular endoplasmic reticulum
Article first published online: 16 NOV 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 2, pages 481–489, January 2013
How to Cite
Lee, H., Sparkes, I., Gattolin, S., Dzimitrowicz, N., Roberts, L. M., Hawes, C. and Frigerio, L. (2013), An Arabidopsis reticulon and the atlastin homologue RHD3-like2 act together in shaping the tubular endoplasmic reticulum. New Phytologist, 197: 481–489. doi: 10.1111/nph.12038
- Issue published online: 18 DEC 2012
- Article first published online: 16 NOV 2012
- Manuscript Accepted: 5 OCT 2012
- Manuscript Received: 29 AUG 2012
- Oxford Brookes Nigel Groome
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Fig. S1 eFP browser expression predictions for RL2 and HVA22b.
Fig. S2 (A) Comparison of the transmembrane topologies of HVA22b and yeast Yop1p, as predicted by TOPCONS (http://topcons.cbr.su.se/) and (B) sequence alignment between Yop1p and HVA22b, generated by ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/).
Fig. S3 Effect of GTPase mutants of RL2 on ER morphology.
Fig. S4 Coexpression of RL2 and RTNLB13 affects the morphology of the cortical ER network.
Fig. S5 (A) Evolutionary relationships of Arabidopsis (At) and human (Hs) derlin proteins; (B) tobacco mesophyll protoplasts were transfected with plasmids encoding the indicated constructs; (C) transmembrane topology of Arabidopsis derlin1, as predicted by TOPCONS (http://topcons.cbr.su.se/).
Fig. S6 Derlin 1-YFP labels, but does not perturb, the ER network.