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Fig. S1 Transposon display and isolation of a fragment involved in the HAM5 petal color.

Fig. S2 Footprints left by the revertants after excision of Tam3 at the sites involved in HAM5 petal color.

Fig. S3 Isolation of the variant that excised upstream Tam3 in the nivbeni allele of HAM5.

Fig. S4 Southern blotting analysis using methylation sensitive restriction enzymes (HpaII/MspI) in the niv promoter region in HAM2 and HAM5.

Fig. S5 Pedigree diagram of the HAM5 lines established in John Innes Center.

Table S1 Primers used for the bisulfite analysis in this study