These authors contributed equally to this work.
The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana
Article first published online: 17 DEC 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 3, pages 815–824, February 2013
How to Cite
Yang, J. L., Chen, W. W., Chen, L. Q., Qin, C., Jin, C. W., Shi, Y. Z. and Zheng, S. J. (2013), The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana. New Phytologist, 197: 815–824. doi: 10.1111/nph.12057
- Issue published online: 7 JAN 2013
- Article first published online: 17 DEC 2012
- Manuscript Accepted: 17 OCT 2012
- Manuscript Received: 7 OCT 2012
- Ministry of Education
- , and by grants from the Innovative Research Team. Grant Number: IRT1185
- Central Universities
- general regulatory factor (GRF);
- iron acquisition;
- nitric oxide (NO);
- plant hormone;
- signal transduction;
- transcription factor
- Here we report the function of a general regulatory factor, GENERAL REGULATORY FACTOR11 (GRF11), in terms of the iron (Fe) deficiency response.
- Physiological and molecular responses of the loss-of-function Arabidopsis thaliana grf11 mutant to Fe supply were investigated. Genes involved in posttranscriptional regulation of FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) were also analyzed. In addition, the molecular link between the signaling molecule nitric oxide (NO) and Fe deficiency responses was further dissected.
- Our results suggest that GRF11 is necessary for induction of Fe-deficiency-tolerance mechanisms. The FIT protein can bind to the promoter of GRF11, which contains an E-box motif. GRF11 also positively affects FIT transcription but has no influence on the genes involved in posttranscriptional regulation of FIT. Furthermore, NO positively regulates GRF11 induction upon the onset of Fe deficiency.
- We propose that, upon the onset of Fe deficiency, induction of FIT expression is dependent on GRF11, which acts downstream of NO to mediate Fe deficiency responses.