The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana

Authors

  • Jian Li Yang,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Wei Wei Chen,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
    Search for more papers by this author
    • These authors contributed equally to this work.
  • Li Qian Chen,

    1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    Search for more papers by this author
  • Cheng Qin,

    1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
    Search for more papers by this author
  • Chong Wei Jin,

    1. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China
    Search for more papers by this author
  • Yuan Zhi Shi,

    1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou, China
    Search for more papers by this author
  • Shao Jian Zheng

    Corresponding author
    • State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
    Search for more papers by this author

Author for correspondence:

Shao Jian Zheng

Tel: +86 571 8820 6438

Email: sjzheng@zju.edu.cn

Summary

  • Here we report the function of a general regulatory factor, GENERAL REGULATORY FACTOR11 (GRF11), in terms of the iron (Fe) deficiency response.
  • Physiological and molecular responses of the loss-of-function Arabidopsis thaliana grf11 mutant to Fe supply were investigated. Genes involved in posttranscriptional regulation of FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) were also analyzed. In addition, the molecular link between the signaling molecule nitric oxide (NO) and Fe deficiency responses was further dissected.
  • Our results suggest that GRF11 is necessary for induction of Fe-deficiency-tolerance mechanisms. The FIT protein can bind to the promoter of GRF11, which contains an E-box motif. GRF11 also positively affects FIT transcription but has no influence on the genes involved in posttranscriptional regulation of FIT. Furthermore, NO positively regulates GRF11 induction upon the onset of Fe deficiency.
  • We propose that, upon the onset of Fe deficiency, induction of FIT expression is dependent on GRF11, which acts downstream of NO to mediate Fe deficiency responses.

Ancillary