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nph12062-sup-0001-FiguresS1-S7-TableS1-S4.docWord document5557K

Fig. S1 Phenotypes and sections of petals used for HPLC analyses.

Fig. S2 Phenotypes, ages, and sections of petals used for qualitative RT-PCR analyses.

Fig. S3 Stages of Clarkia gracilis petal development.

Fig. S4 Genotypes at the P and I loci and expected phenotypes.

Fig. S5 Phylogenetic trees of anthocyanin pathway genes in section Rhodanthos.

Fig. S6 Dihydroflavonol-4-reductase 2 A/B (Dfr2-A/B) expression and Actin control in C. gracilis plants derived from seeds collected in natural populations.

Fig. S7 Alignment of the six different dihydroflavonol-4-reductase (Dfr) sequences found in Clarkia gracilis.

Table S1 Voucher information

Table S2 Primers used in qualitative and quantitative PCR assays

Table S3 Primers used and expected sizes for dihydroflavonol-4-reductase 2 (Dfr2) genotype assays for the P cross

Table S4 Primers used and expected sizes for dihydroflavonol-4-reductase 2 (Dfr2) genotype assays for the I cross

nph12062-sup-0002-NotesS1.txtplain text document26KNotes S1 Text files containing alignments used for phylogenetic analyses.