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Fig. S1 PCR and Southern blot to confirm the deletion of the GcABC-G1.

Fig. S2 Growth curves of Gc and Δgcabc-g1 on MEA with and without monoterpenes.

Fig. S3 Growth rate of Gc and Δgcabc-g1 on MEA with different concentrations of (+)- limonene treatment.

Fig. S4 Individual diterpenes in MEA inhibited the growth of both Gc and Δgcabc-g1 at similar rates.

Fig. S5 Growth of Gc and Δgcabc-g1 with different carbon sources.

Fig. S6 Lodgepole pine stem sections stained by Gc and Δgcabc-g1 at 4 wk after inoculation.

Fig. S7 RNA-seq analysis of differentially expressed genes in Gc and the Δgcabc-g1 mutant in response to a mixture of monoterpenes.

Fig. S8 Functional grouping of genes exclusively upregulated in the Δgcabc-g1 library using InterproScan and KEGG annotation.

Table S1 Subfamily distribution of ABC transporter proteins in various fungal species

Table S2 List of species and proteins used for the phylogenetic analysis of the ascomycota ABC-G group I transporters

Table S3 The fungal intracellular limonene concentration after 1 h and 18 h incubation in Gc and its GcABC-G1 mutant (Δgcabc-g1)

Methods S1 Media composition for fungal growth; measurement of intracellular limonene.