A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees
Article first published online: 17 DEC 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 3, pages 886–898, February 2013
How to Cite
Wang, Y., Lim, L., DiGuistini, S., Robertson, G., Bohlmann, J. and Breuil, C. (2013), A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance to Grosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees. New Phytologist, 197: 886–898. doi: 10.1111/nph.12063
- Issue published online: 7 JAN 2013
- Article first published online: 17 DEC 2012
- Manuscript Accepted: 19 OCT 2012
- Manuscript Received: 28 SEP 2012
- Natural Sciences and Engineering Research Council of Canada
- Genome Canada, Genome BC and Genome Alberta
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Fig. S1 PCR and Southern blot to confirm the deletion of the GcABC-G1.
Fig. S2 Growth curves of Gc and Δgcabc-g1 on MEA with and without monoterpenes.
Fig. S3 Growth rate of Gc and Δgcabc-g1 on MEA with different concentrations of (+)- limonene treatment.
Fig. S4 Individual diterpenes in MEA inhibited the growth of both Gc and Δgcabc-g1 at similar rates.
Fig. S5 Growth of Gc and Δgcabc-g1 with different carbon sources.
Fig. S6 Lodgepole pine stem sections stained by Gc and Δgcabc-g1 at 4 wk after inoculation.
Fig. S7 RNA-seq analysis of differentially expressed genes in Gc and the Δgcabc-g1 mutant in response to a mixture of monoterpenes.
Fig. S8 Functional grouping of genes exclusively upregulated in the Δgcabc-g1 library using InterproScan and KEGG annotation.
Table S1 Subfamily distribution of ABC transporter proteins in various fungal species
Table S2 List of species and proteins used for the phylogenetic analysis of the ascomycota ABC-G group I transporters
Table S3 The fungal intracellular limonene concentration after 1 h and 18 h incubation in Gc and its GcABC-G1 mutant (Δgcabc-g1)
Methods S1 Media composition for fungal growth; measurement of intracellular limonene.