Polymorphic simple sequence repeat (SSR) loci within cellulose synthase (PtoCesA) genes are associated with growth and wood properties in Populus tomentosa
Article first published online: 21 DEC 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 3, pages 763–776, February 2013
How to Cite
Du, Q., Pan, W., Xu, B., Li, B. and Zhang, D. (2013), Polymorphic simple sequence repeat (SSR) loci within cellulose synthase (PtoCesA) genes are associated with growth and wood properties in Populus tomentosa. New Phytologist, 197: 763–776. doi: 10.1111/nph.12072
- Issue published online: 7 JAN 2013
- Article first published online: 21 DEC 2012
- Manuscript Accepted: 27 OCT 2012
- Manuscript Received: 6 SEP 2012
- State Key Basic Research Program of China. Grant Number: 2012CB114506
- Project of the National Natural Science Foundation of China. Grant Numbers: 31170622, 30872042
- candidate-gene-based association mapping;
- Chinese white poplar;
- gene-derived simple sequence repeat (SSR);
- linkage population;
- PtoCesA gene family
- Chinese white poplar (Populus tomentosa), an important commercial tree species for timber and pulp production in northern China, has been used to examine the individual genes and allelic diversity responsible for complex traits controlling growth and lignocellulosic biosynthesis. Taking advantage of the low degree of linkage disequilibrium (LD) within P. tomentosa association populations, we examined associations between 15 cellulose synthase (PtoCesA) genes and traits including growth and wood properties.
- Thirty-six novel simple sequence repeat (SSR) markers within PtoCesA genes were detected by re-sequencing and genotyped in an association population (460 individuals). Single-marker and haplotype-based LD approaches were used to identify significant marker–trait associations. Family-based linkage studies and real-time PCR testing were conducted to validate the functional significance of SSR variation.
- Fifteen single-marker associations from seven PtoCesA genes and nine haplotype-based associations within six genes were identified in the association population (false discovery rate Q < 0.05). Next, five SSR marker–trait associations (Q < 0.05) from four PtoCesA genes were successfully validated in a linkage mapping population (1200 individuals).
- The results imply a functional role for these genes in mediating wood properties, demonstrating the potential of combining single-marker and haplotype-based LD approaches to detect functional allelic variation underlying quantitative traits in a low-LD population.