Indeterminate domain 10 regulates ammonium-mediated gene expression in rice roots

Authors

  • Yuan Hu Xuan,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Ryza A. Priatama,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Jin Huang,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Byoung Il Je,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Jing Miao Liu,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Soon Ju Park,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Hai Long Piao,

    1. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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  • Dae Young Son,

    1. Department of Applied Biology, Gyeongsang National University, Jinju, South Korea
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  • Jeung Joo Lee,

    1. Department of Applied Biology, Gyeongsang National University, Jinju, South Korea
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  • Sung Han Park,

    1. National Academy of Agricultural Science, Rural Development Administration, Suwon, South Korea
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  • Ki Hong Jung,

    1. Crop Biotech Institute, Kyung Hee University, Yongin, South Korea
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  • Tae Ho Kim,

    1. Genomics Division, Department of Agricultural Biotechnology, National Academy of Agricultural Science (NAAS), RDA, Suwon, South Korea
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  • Chang-deok Han

    Corresponding author
    • Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, South Korea
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Author for correspondence:

Chang-deok Han

Tel: +82 55 772 1356

Email: cdhan@gsnu.ac.kr

Summary

  • Indeterminate domain (IDD) genes are a family of plant transcriptional regulators that function in the control of development and metabolism during growth. Here, the function of Oryza sativa indeterminate domain 10 (OsIDD10) has been explored in rice plants. Compared with wild-type roots, idd10 mutant roots are hypersensitive to exogenous ammonium. This work aims to define the action of IDD10 on gene expression involved in ammonium uptake and nitrogen (N) metabolism.
  • The ammonium induction of key ammonium uptake and assimilation genes was examined in the roots of idd10 mutants and IDD10 overexpressors. Molecular studies and transcriptome analysis were performed to identify target genes and IDD10 binding cis-elements.
  • IDD10 activates the transcription of AMT1;2 and GDH2 by binding to a cis-element motif present in the promoter region of AMT1;2 and in the fifth intron of GDH2. IDD10 contributes significantly to the induction of several genes involved in N-linked metabolic and cellular responses, including genes encoding glutamine synthetase 2, nitrite reductases and trehalose-6-phosphate synthase. Furthermore, the possibility that IDD10 might influence the N-mediated feedback regulation of target genes was examined.
  • This study demonstrates that IDD10 is involved in regulatory circuits that determine N-mediated gene expression in plant roots.

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