SHORT INTERNODES/STYLISH genes, regulators of auxin biosynthesis, are involved in leaf vein development in Arabidopsis thaliana
Article first published online: 7 JAN 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 197, Issue 3, pages 737–750, February 2013
How to Cite
Baylis, T., Cierlik, I., Sundberg, E. and Mattsson, J. (2013), SHORT INTERNODES/STYLISH genes, regulators of auxin biosynthesis, are involved in leaf vein development in Arabidopsis thaliana. New Phytologist, 197: 737–750. doi: 10.1111/nph.12084
- Issue published online: 7 JAN 2013
- Article first published online: 7 JAN 2013
- Manuscript Accepted: 30 OCT 2012
- Manuscript Received: 27 AUG 2012
- National Science and Engineering Research Council of Canada
- Swedish Research Council Formas
- SHORT INTERNODES ;
- STYLISH ;
- Leaves depend on highly developed venation systems to collect fixed carbon for transport and to distribute water. We hypothesized that local regulation of auxin biosynthesis plays a role in vein development. To this effect, we assessed the role of the SHORT INTERNODES/STYLISH (SHI/STY) gene family, zinc-finger transcription factors linked to regulation of auxin biosynthesis, in Arabidopsis thaliana leaf vein development.
- Gene functions were assessed by a combination of high-resolution spatio-temporal expression analysis of promoter-marker lines and phenotypic analysis of plants homozygous for single and multiple mutant combinations.
- The SHI/STY genes showed expression patterns with variations on a common theme of activity in incipient and developing cotyledon and leaf primordia, narrowing to apices and hydathode regions. Mutant analysis of single to quintuple mutant combinations revealed dose-dependent defects in vein patterning affecting multiple vein traits, most notably in cotyledons.
- Here we demonstrate that local regulation of auxin biosynthesis is an important aspect of leaf vein development. Our findings also support a model in which auxin synthesized at the periphery of primordia affects vein development.