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Fig. S1 SHI/STY-promoter::GUS expression in developing third rosette leaves.

Fig. S2 Frequencies of secondary vein lower-loop domain (LLD) defects in shi/sty mutant cotyledons and first/second rosette leaves.

Fig. S3 Venation patterns of shi/sty mutant third rosette leaves at 21 DAG.

Fig. S4 Graph illustrating the absence of elongated and interconnected cells in cotyledon ULD gaps.

Table S1 Vascular pattern characteristics of shi/sty mutant cotyledons at 14 DAG

Table S2 Vascular pattern characteristics of shi/sty mutant first/second rosette leaves at 21 DAG