Medicago truncatula DNF2 is a PI-PLC-XD-containing protein required for bacteroid persistence and prevention of nodule early senescence and defense-like reactions
Version of Record online: 27 DEC 2012
© 2012 CNRS. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 4, pages 1250–1261, March 2013
How to Cite
Bourcy, M., Brocard, L., Pislariu, C. I., Cosson, V., Mergaert, P., Tadege, M., Mysore, K. S., Udvardi, M. K., Gourion, B. and Ratet, P. (2013), Medicago truncatula DNF2 is a PI-PLC-XD-containing protein required for bacteroid persistence and prevention of nodule early senescence and defense-like reactions. New Phytologist, 197: 1250–1261. doi: 10.1111/nph.12091
- Issue online: 4 FEB 2013
- Version of Record online: 27 DEC 2012
- Manuscript Accepted: 6 NOV 2012
- Manuscript Received: 3 SEP 2012
- National Science Foundation Plant Genome Research Program. Grant Number: DBI-0703285
- The Samuel Roberts Noble Foundation
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 Medicago truncatula DNF2 is a conserved protein containing a phosphatidylinositol-specific phospholipase C X domain.
Fig. S2 Medicago truncatula dnf2-2, dnf2-3 and dnf2-4 mutants are strongly altered in DNF2 expression.
Fig. S3 dnf2 is required to support nifH expression and nitrogen fixation.
Fig. S4 Occurrence of brownish nodules in dnf2 mutants elicited by Sinorhizobium meliloti Rm41 or Sm1021 strains.
Fig. S5 DNF2 is alternatively spliced in legume plants.
Fig. S6 Impairment of bacteroid differentiation in dnf2 nodules.
Table S1 Primers used in this study
Table S2 dnf2 nodule cells have a reduced endoreduplication index