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Fig. S1 Medicago truncatula DNF2 is a conserved protein containing a phosphatidylinositol-specific phospholipase C X domain.

Fig. S2 Medicago truncatula dnf2-2, dnf2-3 and dnf2-4 mutants are strongly altered in DNF2 expression.

Fig. S3 dnf2 is required to support nifH expression and nitrogen fixation.

Fig. S4 Occurrence of brownish nodules in dnf2 mutants elicited by Sinorhizobium meliloti Rm41 or Sm1021 strains.

Fig. S5 DNF2 is alternatively spliced in legume plants.

Fig. S6 Impairment of bacteroid differentiation in dnf2 nodules.

Table S1 Primers used in this study

Table S2 dnf2 nodule cells have a reduced endoreduplication index