These authors contributed equally to this work.
SlNAC1, a stress-related transcription factor, is fine-tuned on both the transcriptional and the post-translational level
Article first published online: 21 DEC 2012
© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
Volume 197, Issue 4, pages 1214–1224, March 2013
How to Cite
Huang, W., Miao, M., Kud, J., Niu, X., Ouyang, B., Zhang, J., Ye, Z., Kuhl, J. C., Liu, Y. and Xiao, F. (2013), SlNAC1, a stress-related transcription factor, is fine-tuned on both the transcriptional and the post-translational level. New Phytologist, 197: 1214–1224. doi: 10.1111/nph.12096
- Issue published online: 4 FEB 2013
- Article first published online: 21 DEC 2012
- Manuscript Accepted: 11 NOV 2012
- Manuscript Received: 3 OCT 2012
- the National Science Fund Distinguished Young Scholars. Grant Number: No. 2010-6511-42056
- USDA. Grant Numbers: No. 2010-6511-42056, No. 30825030
- NAC transcription factor;
- plant disease resistance;
- tomato (Solanum lycopersicum);
- transcriptional and post-translational regulation;
- ubiquitin–proteasome system (UPS)-mediated degradation
- The plant-specific NAC (NAM, ATAF1,2, CUC2) transcription factors play significant roles in diverse physiological processes. In this study, we determined the regulation of a stress-related tomato (Solanum lycopersicum) NAC1 (SlNAC1) transcription factor at both the transcriptional and the post-translational level.
- The SlNAC1 protein was found to be stable in the presence of proteasome-specific inhibitor MG132 or MG115 and ubiquitinated in plant cells, suggesting that the SlNAC1 is subject to the ubiquitin–proteasome system-mediated degradation. Deletion analysis identified a short segment of 10 amino acids (aa261–270) that was required for ubiquitin–proteasome system-mediated degradation, among which two leucine residues (L268 and L269) were critical for the protein instability of SlNAC1. Fusion of the degron (SlNAC1191–270) containing these 10 amino acids to green fluorescent protein was found to be sufficient to trigger the degradation of the fusion protein.
- In addition, the SlNAC1 gene is strongly upregulated during Pseudomonas infection, while repression of the NAC1 ortholog in Nicotiana benthamiana resulted in enhanced susceptibility to Pseudomonas bacteria.
- These results suggest that rapid upregulation of the NAC1 gene resulting in more protein production is likely one of the strategies plants use to defend themselves against pathogen infection.