Resistance to herbicides caused by single amino acid mutations in acetyl-CoA carboxylase in resistant populations of grassy weeds
Article first published online: 10 JAN 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 197, Issue 4, pages 1110–1116, March 2013
How to Cite
Jang, S., Marjanovic, J. and Gornicki, P. (2013), Resistance to herbicides caused by single amino acid mutations in acetyl-CoA carboxylase in resistant populations of grassy weeds. New Phytologist, 197: 1110–1116. doi: 10.1111/nph.12117
- Issue published online: 4 FEB 2013
- Article first published online: 10 JAN 2013
- Manuscript Accepted: 25 NOV 2012
- Manuscript Received: 13 NOV 2012
- fatty acid biosynthesis;
- grassy weeds;
- herbicide resistance;
- plant acetyl-CoA carboxylase
- Eleven spontaneous mutations of acetyl-CoA carboxylase have been identified in many herbicide-resistant populations of 42 species of grassy weeds, hampering application of aryloxyphenoxypropionate, cyclohexadione and phenylpyrazoline herbicides in agriculture.
- IC50 shifts (resistance indices) caused by herbicide-resistant mutations were determined using a recombinant yeast system that allows comparison of the effects of single amino acid mutations in the same biochemical background, avoiding the complexity inherent in the in planta experiments. The effect of six mutations on the sensitivity of acetyl-CoA carboxylase to nine herbicides representing the three chemical classes was studied.
- A combination of partially overlapping binding sites of the three classes of herbicides and the structure of their variable parts explains cross-resistance among and between the three classes of inhibitors, as well as differences in their specificity. Some degree of resistance was detected for 51 of 54 herbicide/mutation combinations.
- Introduction of new herbicides targeting acetyl-CoA carboxylase will depend on their ability to overcome the high degree of cross-resistance already existing in weed populations.