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Fig. S1 Chemical structure of CoA and nine herbicides used in this study.

Fig. S2 Resistance index for six ACCase mutations and cytosolic ACCase relative to wheat plastid ACCase.

Table S1 Yeast strain doubling times and IC50 values

Methods S1 Preparation of yeast ACCase gene-replacement strains, analysis of ACCase inhibition by herbicides in yeast gene-replacement strains, in planta (wheat leaves) and in vitro.