Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 is a negative regulator of cold acclimation
Article first published online: 16 JAN 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 1, pages 95–102, April 2013
How to Cite
Sasaki, K., Kim, M.-H. and Imai, R. (2013), Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 is a negative regulator of cold acclimation. New Phytologist, 198: 95–102. doi: 10.1111/nph.12118
- Issue published online: 25 FEB 2013
- Article first published online: 16 JAN 2013
- Manuscript Accepted: 27 NOV 2012
- Manuscript Received: 10 OCT 2012
- the Japan Society for the Promotion of Science. Grant Numbers: 22380063, 19380063
- the National Agriculture and Food Research Organization. Grant Number: 3204
- cold acclimation;
- cold shock domain;
- flowering time;
- freezing tolerance;
- RNA chaperone;
- silique development
- Bacterial cold shock proteins (CSPs) act as RNA chaperones that destabilize mRNA secondary structures at low temperatures. Bacterial CSPs are composed solely of a nucleic acid-binding domain termed the cold shock domain (CSD). Plant CSD proteins contain an auxiliary domain in addition to the CSD but also show RNA chaperone activity. However, their biological functions are poorly understood.
- We examined Arabidopsis COLD SHOCK DOMAIN PROTEIN 2 (AtCSP2) using overexpressing and mutant lines.
- A double mutant, with reduced AtCSP2 and no AtCSP4, showed higher freezing tolerance than the wild-type when cold-acclimated. The increase in freezing tolerance was associated with up-regulation of CBF transcription factors and their downstream genes. By contrast, overexpression of AtCSP2 resulted in decreased freezing tolerance when cold-acclimated. In addition, late flowering and shorter siliques were observed in the overexpressing lines.
- AtCSP2 negatively regulates freezing tolerance and is partially redundant with its closest paralog, AtCSP4.