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nph12120-sup-0001-FigsS1-S6.pptxapplication/pptx3029K

Fig. S1 Validation of the selected biological conditions for MtEnod11 gene expression and reactive oxygen species (ROS) levels.

Fig. S2 Comparison of expression ratios of probesets in RT-qPCR and Affymetrix gene array analyses. Analysis, RT-qPCR, of the differential accumulation of transcripts, initially identified by Affymetrix GeneChip analysis (Table S2), on two new biological replicates (data from Table 1).

Fig. S3 HyPer protein production in composite plants of Medicago truncatula.

Fig. S4 Relative expression of selected genes in untreated Medicago truncatula roots.

Fig. S5 Phenotypes of infection threads in artificial micro-RNA (amiRNA) MtSpk1 plants.

Fig. S6 Subcellular distribution of MtSPK1.

nph12120-sup-0002-TablesS1-S4.xlsapplication/msexcel270K

Table S1 Primers used for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, MtSpk1 promoter, open reading frame (ORF) and artificial micro-RNA (amiRNA) cloning

Table S2 Medicago truncatula genes regulated 2 d after inoculation

Table S3 Genes expressed in the meristematic or infection zone of Medicago truncatula nodules

Table S4 Reactive oxygen species (ROS)-regulated genes of Arabidopsis and Medicago truncatula

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.