Differential induction of mitochondrial machinery by light intensity correlates with changes in respiratory metabolism and photorespiration in rice leaves
Article first published online: 29 JAN 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 1, pages 103–115, April 2013
How to Cite
Huang, S., Jacoby, R. P., Shingaki-Wells, R. N., Li, L. and Millar, A. H. (2013), Differential induction of mitochondrial machinery by light intensity correlates with changes in respiratory metabolism and photorespiration in rice leaves. New Phytologist, 198: 103–115. doi: 10.1111/nph.12123
- Issue published online: 25 FEB 2013
- Article first published online: 29 JAN 2013
- Manuscript Accepted: 27 NOV 2012
- Manuscript Received: 5 OCT 2012
- ARC Centre of Excellence in Plant Energy Biology. Grant Numbers: CE0561495, FT110100242
- University International Stipend
- Grains Research and Development Corporation
- Australian Postgraduate Award PhD scholarship. Grant Number: FT110100242
- University of Western Australia and Agilent Technologies Australia
- Centre for Comparative Analysis of Biomolecular Networks
- ARC Australian Future Fellow. Grant Number: FT110100242
- Scholarship International Research Fees
- a Top Up Scholarship
- rice (Oryza sativa)
- The light responsiveness of mitochondrial function was investigated through changes in mitochondrial composition and metabolism in rice (Oryza sativa) shoots.
- The mitochondrial proteome and metabolite abundances under low light, (LL, 100 μmol m−2 s−1), and high light (HL, 700 μmol m−2 s−1) were measured along with information on shoot photosynthetic, respiratory and photorespiratory activity.
- Specific steps in mitochondrial tricarboxylic acid (TCA) cycle metabolism were decreased under HL, correlating with lower respiration rate under HL. The abundance of mitochondrial enzymes in branch chain metabolism was reduced under HL/LL, and correlated with a decrease in the abundance of a range of amino acids in the HL/LL. Mitochondrial nucleoside diphosphate kinase was increased under LL/HL treatments. Significant accumulation of glycine decarboxylase P, T subunits and serine hydroxymethyltransferase occurred in response to light. The abundance of the glycine decarboxylase (GDC) H subunit proteins was not changed by HL/LL treatments, and the abundance of GDC L subunit protein was halved under HL, indicating a change in the stoichiometry of GDC subunits, while photorespiration was fourfold higher in LL- than in HL-treated plants.
- Insights into these light-dependent phenomena and their importance for understanding the initiation of photorespiration in rice and adaptation of mitochondria to function in photosynthetic cells are discussed.