These authors contributed equally to this work.
Short-chain chitin oligomers from arbuscular mycorrhizal fungi trigger nuclear Ca2+ spiking in Medicago truncatula roots and their production is enhanced by strigolactone
Article first published online: 6 FEB 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 1, pages 190–202, April 2013
How to Cite
Genre, A., Chabaud, M., Balzergue, C., Puech-Pagès, V., Novero, M., Rey, T., Fournier, J., Rochange, S., Bécard, G., Bonfante, P. and Barker, D. G. (2013), Short-chain chitin oligomers from arbuscular mycorrhizal fungi trigger nuclear Ca2+ spiking in Medicago truncatula roots and their production is enhanced by strigolactone. New Phytologist, 198: 190–202. doi: 10.1111/nph.12146
- Issue published online: 25 FEB 2013
- Article first published online: 6 FEB 2013
- Manuscript Accepted: 5 DEC 2012
- Manuscript Received: 15 OCT 2012
- Converging Technologies project funded by Regione Piemonte
- Italian National project PRIN 2008
- French National Research Agency. Grant Number: ANR-08-BLAN-0029-01
- French National Centre Scientific Research. Grant Number: PICS 4267
- French Ministry of Research and Higher Education
- arbuscular mycorrhiza (AM);
- chitin oligomers;
- common SYM pathway;
- fungal–plant signalling;
- germinated spore exudates;
- Medicago truncatula ;
- nuclear calcium spiking;
- Top of page
- Materials and Methods
- Supporting Information
- The primary objective of this study was to identify the molecular signals present in arbuscular mycorrhizal (AM) germinated spore exudates (GSEs) responsible for activating nuclear Ca2+ spiking in the Medicago truncatula root epidermis.
- Medicago truncatula root organ cultures (ROCs) expressing a nuclear-localized cameleon reporter were used as a bioassay to detect AM-associated Ca2+ spiking responses and LC-MS to characterize targeted molecules in GSEs.
- This approach has revealed that short-chain chitin oligomers (COs) can mimic AM GSE-elicited Ca2+ spiking, with maximum activity observed for CO4 and CO5. This spiking response is dependent on genes of the common SYM signalling pathway (DMI1/DMI2) but not on NFP, the putative Sinorhizobium meliloti Nod factor receptor. A major increase in the CO4/5 concentration in fungal exudates is observed when Rhizophagus irregularis spores are germinated in the presence of the synthetic strigolactone analogue GR24. By comparison with COs, both sulphated and nonsulphated Myc lipochito-oligosaccharides (LCOs) are less efficient elicitors of Ca2+ spiking in M. truncatula ROCs.
- We propose that short-chain COs secreted by AM fungi are part of a molecular exchange with the host plant and that their perception in the epidermis leads to the activation of a SYM-dependent signalling pathway involved in the initial stages of fungal root colonization.
- Top of page
- Materials and Methods
- Supporting Information
Arbuscular mycorrhizal (AM) associations are ancient plant symbioses playing a central role in most land ecosystems, both by improving soil nutrient uptake and by contributing to protection against fungal pathogens. It has been proposed that the establishment of the AM endosymbiosis requires a molecular dialogue between the plant and fungal partners. Strong evidence suggests that a key component of this dialogue is the perception of host-secreted strigolactones, which activate fungal development and, in particular, the intense hyphal ramification in the vicinity of the root which precedes colonization of the root tissue (Akiyama et al., 2005; Besserer et al., 2006). However, the obligate biotrophy of AM fungi and the inability to perform genetics on these microbes have greatly hampered the characterization of the reciprocal fungal signalling molecules. Most of our current knowledge on symbiotic signalling derives from parallel studies on the Rhizobium–legume symbiosis, where it has been shown that these nitrogen-fixing bacteria synthesize specific decorated lipochito-oligosaccharides (LCOs) termed Nod factors (Dénarié & Cullimore, 1993). In addition, plant genetics has led to the characterization of several genes essential for initiating both root endosymbioses (MtDMI1, MtDMI2 and MtDMI3 in the case of the model legume Medicago truncatula), thus defining a shared ‘common SYM’ signalling pathway (Parniske, 2008). This pathway also relies on calcium-mediated signalling, and in particular the triggering of nuclear-associated Ca2+ spiking (Oldroyd & Downie, 2006). Upstream of the common SYM pathway, perception of Nod factors requires the MtNFP gene, encoding a LysM Receptor-Like Kinase (Arrighi et al., 2006). However, although NFP plays some role in host responses to Myc LCOs (Maillet et al., 2011; Czaja et al., 2012), it is not essential for AM colonization, since nfp mutants have a wildtype (WT) AM pheno-type (Ben Amor et al., 2003).
Since the bacterial symbiosis evolved more recently than the AM association (60 vs 450 million yr ago), it is currently thought that rhizobia have exploited the ancient mycorrhizal signalling pathway by mimicking symbiotic fungal signals. Therefore, by analogy with bacterial Nod factors, it has been proposed that AM fungi release signal molecules (Myc factors) essential for recognition of the fungal partner (Albrecht et al., 1999; Catoira et al., 2000). This has led to the hypothesis that modern legumes possess two separate branches that feed into the common SYM pathway, one for each type of root symbiont. Thus, hypothetical AM Myc factors should be able to elicit SYM pathway-dependent early plant responses that are specific to mycorrhizal colonization and their activity is likely to be independent on the NFP Nod factor receptor. In addition, and in contrast to Nod factors, Myc factors should be active on a wide variety of plant hosts (including, of course, nonlegumes), as there is no evidence for strict host specificity in the AM association.
A number of studies have indicated the production of diffusible fungal signals by either AM hyphae, germinating spores or mycorrhizal roots. The responses to these fungal signals include changes in intracellular calcium concentration (Navazio et al., 2007; Chabaud et al., 2011) and gene transcriptional activation (Kosuta et al., 2003; Weidmann et al., 2004; Drissner et al., 2007; Kuhn et al., 2010; Mukherjee & Ané, 2011; Ortu et al., 2012). Not all of these responses are dependent on the common SYM pathway, suggesting the existence of alternative signalling pathways and possibly also a variety of fungal factors. Recently, LCOs have been identified at low concentrations in mycorrhizal root exudates and in germinating AM spore exudates using bioassays originally established for Nod factor characterization (Maillet et al., 2011). These so-called Myc LCOs are active in stimulating lateral root development and overall levels of mycorrhization. Furthermore, genetic studies have shown that, as for Nod factors, enhanced lateral root development in response to Myc LCOs is SYM pathway-dependent. Finally, this biological response is totally or partially dependent upon the NFP gene depending on the Myc LCO concentration.
Since Ca2+ signalling in the form of sustained intracellular oscillations is a central feature of the common SYM pathway, in vivo fluorescent Ca2+ reporters known as cameleons have proved to be particularly useful tools for evaluating cellular responses to microbial symbiotic signals in the host epidermis (Miwa et al., 2006; Kosuta et al., 2008). Purified rhizobial Nod factors elicit characteristically regular Ca2+ spiking in both the nucleus and cytoplasm of M. truncatula root hairs (Ehrhardt et al., 1996; Sieberer et al., 2009), whereas germinated spore exudates (GSEs) of the AM fungus Gigaspora margarita trigger nonregular spiking in root epidermal atrichoblasts of the same legume as well as in the nonlegume Daucus carota (Chabaud et al., 2011). Importantly, AM GSEs are active on M. truncatula root organ cultures (ROCs), consistent with the fact that ROCs can be successfully colonized by AM fungi. Furthermore, this AM-elicited calcium signalling is dependent on the common SYM genes DMI1 and DMI2 and independent of NFP. By contrast, rhizobia are unable to nodulate ROCs, and Sinorhizobium meliloti Nod factors fail to induce Ca2+ spiking in the ROC epidermis (Chabaud et al., 2011).
In this article, we have made use of ROCs expressing the nuclear-localized Nup-YC2.1 cameleon as a Nod-independent bioassay to identify and characterize potential symbiotic signal molecules of AM fungal origin. We have discovered that the nonregular spiking observed in response to G. margarita GSEs (Chabaud et al., 2011; this study) is also observed in response to exudates of G. rosea and the distantly related glomeromycete Rhizophagus irregularis. By contrast, GSEs of the biotrophic pathogen Colletotrichum trifolii are unable to trigger spiking in the M. truncatula ROC epidermis. The ROC/cameleon bioassay, coupled to mass spectrometric analysis, have together revealed that short-chain chitin oligomers (COs) elicit an analogous nuclear spiking signature and furthermore are present in the AM GSEs. Importantly, this CO-activated Ca2+ signalling is SYM pathway-dependent and NFP-independent. The addition of the synthetic strigolactone GR24 results in a striking increase both in the CO concentrations in R. irregularis GSEs and in the Ca2+ spiking activity of these exudates. These findings lead to the proposal that short-chain chitin oligomers generated by AM fungi are novel signals perceived in epidermal tissues of host plants and capable of activating an AM-related signalling pathway.
Materials and Methods
- Top of page
- Materials and Methods
- Supporting Information
Medicago truncatula Gaertn. genotype Jemalong A17 and the derived mutants nfp-2 (Arrighi et al., 2006), dmi1-1 and dmi2-2 (Catoira et al., 2000; Wais et al., 2000) were used in this study. M. truncatula ROCs expressing the 35S:NupYC2.1 construct (Sieberer et al., 2009) were obtained previously (Chabaud et al., 2011) and propagated in vertical dishes to favour a regular fishbone-shaped root system (Chabaud et al., 2002). Wildtype A17 and mutant nfp-2 composite plants were obtained following transformation with Agrobacterium rhizogenes according to Boisson-Dernier et al. (2001). Two weeks after transformation, composite plantlets were transferred to vertical square dishes (12 × 12 cm²) containing M medium (Bécard & Fortin, 1988) without sucrose, and covered with Biofolie 25™ (Lumox, Sarstedt, France) as described in Genre et al. (2005), and with the lower part of the dish wrapped in black plastic to protect the root from light. Roots from composite plants were used following 11–15 d of culture on M medium without sucrose.
Nongravitropic ROCs from the horticultural Daucus carota var. sativus expressing the 35S:NupYC2.1 were obtained previously (Chabaud et al., 2011) according to Bécard & Fortin (1988) and were grown horizontally. The Arabidopsis thaliana ecotype Columbia was transformed by flower dipping with A. tumefaciens strain GV3101 carrying the 35S:NupYC2.1 construct according to Clough & Bent (1998). Transformed T1 and T2 progenies were selected on 50 mg l−1 kanamycin. Seeds from a single T2 line with high-level fluorescence were surface-sterilized and grown on M medium without sucrose in vertical dishes, with the lower part wrapped in black plastic. Lateral roots from 9-d-old plantlets were used for the test with chitin oligomers.
Fungal materials and preparation of germinated spore exudates
The AM fungi used in this study were Gigaspora margarita (BEG 34, International Bank for the Glomeromycota, University of Kent, UK), Gigaspora rosea (DAOM 194757, National Mycological Herbarium, Ottawa, Canada) and Rhizophagus irregularis (previously called Glomus intraradices (DAOM 197198); Krüger et al., 2012). Germinated spore exudates (GSEs) from G. margarita and G. rosea were prepared as described in Chabaud et al. (2011). Batches of 100 surface-sterilized G. margarita or G. rosea spores were placed in 1 ml of sterile H2O and incubated in the dark for 7 d at 30°C (2% CO2) to induce germination (germination rate > 90%). The GSE was recovered by pipetting, concentrated 10-fold using a lyophilizer and then stored at −20°C. One hundred microlitre aliquots (equivalent to 100 spores) were used in the ROC Ca2+ spiking bioassay. Chitinase treatment of the G. margarita GSE was performed using 1 mg ml−1 chitinase from Streptomyces griseus (ref C6137; Sigma-Aldrich) in sterile H2O for 16 h at room temperature. This enzyme complex degrades chitin to its monomer subunits.
To prepare R. irregularis GSEs, 125 × 103 sterile spores (Agronutrition, Labège, France) were placed in 40 ml sterile H2O and incubated for 7 d in the dark at 30°C (2% CO2). When GSEs were prepared in the presence of strigolactone, GR24 was added to a final concentration of either 10−6 or 10−8 M. After incubation, the spores were removed by filtration (0.45 μm nitrocellulose filters; Millipore) and a butanol extraction performed on the GSE. The aqueous phase was lyophilized and resuspended in 100 μl H2O. Mass spectrometric analysis was performed on a 10 μl aliquot (400-fold concentrated compared with the GSE – equivalent to 12.5 × 103 spores), and the same quantity used in the ROC Ca2+ spiking bioassay after diluting 10-fold in H2O (40-fold concentrated compared with the GSE).
Colletotrichum trifolii spores were prepared as described in Torregrosa et al. (2004). A total of 107 spores were diluted in 100 ml of sterile H2O. After 24 h incubation at 24°C, the germinated spores were pelleted by centrifugation at 5000 g for 15 min and the GSE was recovered for analysis. C. trifolii GSEs were lyophilized and resuspended in 400 μl H2O. Mass spectrometry analysis was performed directly using 10 μl of the GSE (250-fold concentrated – equivalent to 2.5 × 105 spores) and the ROC/Ca2+ spiking bioassay using 100 μl of the GSE concentrate.
Bioassays for Ca2+ spiking using roots expressing the NupYC2.1 reporter
Several cm-long young lateral root segments (second-order) from either ROCs or composite plants, or young third-order lateral roots grown under Biofolie membrane (to limit root hair development), were excised and placed in a microchamber and treated with 100 μl of the solution to be tested as described in Chabaud et al. (2011). In the case of Arabidopsis, the entire plantlet was placed on the slide, with the whole root system in the microchamber. Confocal FRET-based ratio imaging for detecting and plotting relative changes of nuclear Ca2+ concentrations corresponding to yellow fluorescent protein (YFP) to cyan fluorescent protein (CFP) fluorescence intensity changes over time (Miyawaki et al., 1997) was performed according to Sieberer et al. (2009) using a Leica TCS SP2 AOBS confocal laser-scanning microsope equipped with a long-distance HCX Apo L NA 40X 0.80 water-immersion objective or a HCX PL APO 40X 0.85 dry objective (Leica Microsystems GmbH, Wetzlar, Germany). Fluorescence intensity for both the CFP and YFP moieties of NupYC2.1 were collected, setting the pinhole diameter at 4–5 Airy units, exciting the probe at 458 nm (80% Ar laser) and recording the emitted fluorescence at 470–500 and 530–570 nm, respectively. Transmitted-light images were acquired simultaneously to confirm cell identity. Images were scanned at a resolution of 512 × 512 pixels and collected every 5 s over a period of 30 min after treatment. Average YFP and CFP fluorescence intensities were calculated for each nucleus using Leica LCS or Image J software (NIH, Bethesda, MD, USA). Values were then exported to a Microsoft Excel spreadsheet in order to calculate the YFP/CFP ratio for each time frame. Ratio values were then plotted over time to obtain a graphical representation of FRET intensity, corresponding to relative Ca2+ concentration variations in the nucleoplasm. As previously reported (Chabaud et al., 2011), nuclear spiking in atrichoblasts was never observed in control untreated roots or before the addition of GSEs or chitin-based elicitors. Ca2+ peaks were then counted manually and frequency distribution histograms of peak numbers were prepared as shown in Fig. 3 and Supporting Information Fig. S2. The total numbers of epidermal cell nuclei and independent roots analysed for each test are presented in Table S1.
Biochemicals used for bioassays
Short-chain chitin oligomers were purchased from either Yaizu Suisankagaku Industry Corporation (Tokyo, Japan) or Seikagaku Biobusiness Corporation (Tokyo, Japan), and in addition CO3 and CO4 were kindly provided by Klaus Tietjen (Bayer Crop Science, Monheim, Germany). CO8 was kindly provided by Dr Naoto Shibuya from Meiji University, Kawasaki, Japan. The structure and purity of the commercial chitin oligomers were confirmed by MRM analysis, using two MRM transitions for each CO (see the following section). These analyses revealed that each CO was uncontaminated by either longer- or shorter-length COs and that there were no traces of any LCOs. In addition, the COs were fully N-acetylated. Stock solutions of chitin oligomers were prepared in H2O at 10−3 M and stored at −20°C. S. meliloti Nod factor solutions were freshly prepared from a concentrated 10−3 M stock (ethanol : water (1 : 1 (v/v))). Both sulphated and nonsulphated Myc LCOs were kindly provided by Eric Samain, Hugues Driguez, Sébastien Fort (CNRS, Grenoble, France) and Fabienne Maillet, Jean Dénarié (LIPM, Castanet-Tolosan, France). Myc LCOs were freshly prepared as C16:1 : C18:0 (1 : 1) mixtures from concentrated 10−4 M stocks (acetonitrile : water (1 : 1 (v/v))). In the case of nonsulphated Myc LCOs, dilutions were performed in such a way as to ensure that the final acetonitrile concentration was at least 0.5%. Finally, biochemical analysis was used to confirm the concentrations of the less water-soluble molecules (CO8 and nonsulphated LCOs) used in the bioassays.
Biochemical analyses of GSEs
Chito-oligosaccharides were detected using a 4000 Q Trap mass spectrometer with a Turbo V ESI source in the positive mode, coupled to an Agilent 1100 series HPLC system (Agilent Technologies Inc., Santa Clara, CA, USA). High-performance liquid chromatography (HPLC) separation was performed using a hypercarb column (5 μm, 2 × 100 mm; Hypercarb, Thermo; Suginta et al., 2009). For HPLC analysis, 10 μl of GSE solutions was directly injected. Solutions of acetic acid : water (1 : 103 (v/v); A) and acetic acid : acetonitrile (1 : 103 (v/v); B) were pumped at 0.3 ml min−1. The gradient used was from 100% A to 100% B in 30 min.
The COs were identified in the MRM mode by monitoring the transitions from parent ion > common daughter ions (m/z: 204, 407, 610), and for quantification using the MRM transitions 628 > 204 m/z (CO3), 831 > 204 m/z (CO4), 1034 > 204 m/z (CO5), 1237 > 204 m/z (CO6), 1643 > 204 m/z (CO8). As an example, Fig. S1 illustrates a comparison of the MS/MS spectra for the CO4 standard and CO4 present in the AM fungal GSE. In addition, Table S2 compares the observed retention times and corresponding parent ions for short-chain CO standards and the GSE COs. Note that since retention times can vary slightly between experimental sessions, CO standards were always injected before and after the GSE samples. Blank controls confirmed the absence of residual signal carryover.
In order to determine the concentration of COs in the GSEs, a CO4 standard was added to the GSE before analysis. Since the CO4 signal obtained was threefold lower than expected due to the complexity of the matrix, this additional factor was integrated for determining the true concentrations of COs present within the GSEs.
- Top of page
- Materials and Methods
- Supporting Information
Short-chain chitin oligomers mimic AM exudate-elicited Ca2+ spiking activity in the host root epidermis
In order to determine to what extent the intracellular Ca2+ signalling previously observed in the host root epidermis in response to G. margarita spore exudates (Chabaud et al., 2011) could be generalized to other AM fungal species, we isolated GSEs both from a second Gigaspora species (G. rosea) and from the distantly related R. irregularis. Fig. 1 shows that sustained Ca2+ spiking could be observed in M. truncatula ROC atrichoblasts expressing the nuclear-localized cameleon NupYC2.1 when treated with either the Gigaspora or Rhizophagus GSEs. In all cases, the spiking profiles were highly irregular in terms of both the periodicity and the total number of spikes recorded over the 30 min period following the addition of the exudates. No correlation could be established between the AM species and the Ca2+ spiking profile, which exhibited considerable cell-to-cell variation as previously reported for G. margarita (Chabaud et al., 2011).
In order to characterize the bioactive molecules released by germinating AM fungal spores and responsible for triggering nonregular Ca2+ spiking in the host epidermis, we initially evaluated the chitinase-sensitivity of the G. margarita exudate. Fig. 2(a) shows that chitinase treatment completely abolished the spiking response, thus indicating a chitin-based structure for the putative symbiotic fungal factors. As part of a screen for potential candidate signals, initial tests performed on a mixture of chitin oligomers (COs) comprising 1–6 N-acetyl glucosamine residues revealed that the CO mix (10−8 M) was sufficient to activate sustained nonregular nuclear Ca2+ spiking in ROC epidermal cells (Fig. 2b). Subsequent experiments performed with the identical concentration of individual chitin oligomers (CO3–6; Fig. 2c–f) showed that maximal Ca2+ spiking was obtained with chitotetraose and chitopentaose (see Fig. S2a for a semiquantitative analysis). In all cases, the Ca2+ spiking profiles closely resembled the nonregular fungal GSE-elicited spiking. Finally, although all the Ca2+ responses presented in Fig. 2(a–f) were measured in ROC atrichoblast cells, short-chain COs also triggered nonregular spiking in root hair nuclei, as previously shown for G. margarita GSEs (Chabaud et al., 2011).
Having established that short-chain COs were able to elicit nonregular Ca2+ spiking in M. truncatula ROCs, we then examined whether this response requires activation of the common SYM pathway. The fact that spiking failed to be elicited in either dmi1 or dmi2 mutant lines after addition of 10−8 M CO4 (Fig. 2g,h) implies that the common SYM pathway is indeed required for signal transduction upstream of nuclear Ca2+ signalling. Furthermore, our experiments also revealed that CO-elicited spiking was unaltered in an nfp mutant background (Fig. 2i). Thus, as for the G. margarita GSE, CO perception in ROCs is not dependent on the putative Nod factor receptor NFP.
In order to evaluate Ca2+ spiking responses to short-chain chitin oligomers in nonlegumes, we made use of available D. carota ROC lines expressing Nup-YC2.1 (Chabaud et al., 2011). We had previously observed that G. margarita GSEs were able to activate spiking in carrot roots (Chabaud et al., 2011), and Fig. 2(j) shows that 10−8 M CO4 is also an efficient trigger of Ca2+ spiking in this AM host. We then introduced the same nuclear-localized cameleon into the nonAM host A. thaliana (see the 'Materials and Methods' section). Significantly, chitotetraose failed to elicit spiking in the root epidermis of A. thaliana plants (Fig. 2k).
Finally, in order to compare Ca2+ spiking responses between short-chain COs and longer COs known to elicit plant defence responses (Shibuya & Minami, 2001; Shimizu et al., 2010), we treated M. truncatula ROCs with 10−8 M chitooctaose. Figs 2(l) and S2 show that CO8 is a very poor elicitor of spiking in Medicago roots, with responses even lower than those observed with CO6. In conclusion, short-chain chitin oligomers (CO4/5 are the most active) are able to trigger nonregular Ca2+ spiking in ROCs of AM host plants via the common SYM pathway and independently of NFP.
Strigolactone boosts short-chain CO concentration in AM germinated spore exudates
Having shown that short-chain COs elicit similar Ca2+ spiking responses in epidermal tissues of AM hosts as compared with AM fungal GSEs, we then investigated the chitin oligomer composition of these exudates (see the 'Materials and Methods' section). Typical profiles obtained by multiple reaction monitoring (MRM) for CO3, CO4, CO5 and CO6 species are shown in Fig. S3. Quantitative data corresponding to the peak areas for each chitin oligomer are presented in Table 1, and reveal broadly similar concentrations for the four chitin oligomers. When these values are converted to concentrations using CO standards (see the 'Materials and Methods' section), our data reveal that CO3–6 are each present in the R. irregularis GSE in the concentration range 0.3–1.2 × 10−9 M. Since the Ca2+ spiking experiments were performed with 40× concentrated GSEs, this means that the most active short-chain COs are present at a total concentration of c. 5 × 10−8 M. Similar results were obtained for GSEs of G. rosea (e.g. 0.9 × 10−9 M for CO4), although detailed analyses were more difficult to perform for Gigaspora species because of the limited availability of spores. Thus, the amounts of short-chain COs present in the various AM GSEs used in the Ca2+ spiking bioassay are consistent with the spiking profiles obtained with purified 10−8 M COs (Fig. 2).
|GSE − GR24 (counts × 10−3)||GSE + GR24 (counts × 10−3)||GR24 stimulation|
|CO3||33.5 ± 1.5||154 ± 7.0||× 4.6|
|CO4||44.7 ± 2.3||1690 ± 95||× 38|
|CO5||28.7 ± 1.7||1970 ± 120||× 69|
|CO6||17.0 ± 5.6||263 ± 18||× 15|
In the case of the endosymbiotic rhizobial/legume association, the perception of secreted plant flavonoids by the soil bacterium is essential for optimizing Nod factor synthesis before root infection. To date, strigolactones are the only signalling components of host origin found to be essential for the AM symbiosis (Gomez-Roldan et al., 2008; Kretzschmar et al., 2012). We therefore examined whether the addition of the synthetic strigolactone GR24 modified the concentrations of secreted COs present in the AM GSEs. The inclusion of 10−6 M GR24 in the germination medium of R. irregularis spores (see the 'Materials and Methods' section) led to a major increase (c. 50-fold) in the concentrations of both chitotetraose and chitopentaose (Table 1). On the other hand, the increase was less important (five- to 15-fold) for the CO3 and CO6 species. In terms of nuclear Ca2+ spiking activity, the positive effect of strigolactone can be seen by comparing the responses observed in atrichoblasts treated with GSEs prepared in either the absence or presence of 10−6 M GR24 (Fig. S4). The major stimulation in short-chain chitin oligomer levels correlates with significantly enhanced spiking over the 30 min observation period. Indeed, when we add increasing concentrations of CO4 (up to 10−6 M) to M. truncatula ROCs, we observe an increase in the percentage of spiking atrichoblasts and also an overall increase in spiking intensity (not shown). In addition, it should be underlined that CO3–6 concentrations in the R. irregularis GSE were still enhanced even when the GR24 concentration was lowered to 10−8 M, but in this case increases were limited to between three and eightfold (not shown). By contrast, when 10−6 M GR24 was included in the germination medium of G. rosea spores, we observed only modest increases (three- to fourfold) in short-chain CO concentrations. Finally, we were unfortunately unable to detect any LCOs in the butanol fractions of the R. irregularis GSEs tested. We presume that this is due to the low concentrations of Myc LCOs present in these GSEs (Maillet et al., 2011) and the fact that the MS sensitivity for the detection of LCOs is c. 100-fold lower than that for COs.
Germinated spore exudates from a pathogenic fungus do not elicit Ca2+ spiking in ROCs
If, as these data suggest, the short-chain COs present in AM GSEs are primarily responsible for epidermal Ca2+ spiking responses in M. truncatula roots, exudates from other chitin-rich biotrophic fungi would also be expected to elicit spiking in the ROC bioassay. To evaluate this, we prepared exudates from germinated spores of the M. truncatula hemibiotrophic pathogenic fungus Colletotrichum trifolii (see the 'Materials and Methods' section). Mass spectrometric analysis revealed that CO4 was indeed present at similar concentrations in these exudates compared with AM GSEs (not shown). However, to our surprise, C. trifolii exudates failed to elicit any Ca2+ spiking in the M. truncatula root epidermis and furthermore a negative response was obtained even when the exudate was supplemented with 10−8 M purified CO4 (Fig. S5). We therefore conclude that components of the pathogenic fungal exudate must be inhibiting the CO-elicited epidermal spiking.
Short-chain COs are more active than LCOs in eliciting NFP-independent Ca2+ spiking
Having shown that short-chain COs are efficient elicitors of NFP-independent Ca2+ spiking in M. truncatula ROCs, it was important to examine the activities of other known chitin-based symbiotic signals. We had previously demonstrated that 10−8 M S. meliloti Nod factors were unable to trigger spiking in ROCs (Chabaud et al., 2011), and this result is presented in histogram format in Fig. 3. Indeed, even at the much higher concentration of 10−5 M, these sulphated LCOs elicited only a limited spiking response (Fig. 3). Strikingly, this Nod factor-elicited spiking in the ROC epidermis resembles the nonregular Ca2+ spiking triggered by AM GSEs and COs, and furthermore this response is NFP-independent (Fig. S6a,b). Together, this suggests that the identical signalling pathway is being activated by both 10−8 M COs and the 103-fold higher concentrations of sulphated LCO.
Recently, Maillet et al. (2011) have described several LCO species present in low concentrations in exudates of AM-colonized carrot roots and in GSEs of R. irregularis. These LCOs are N-acylated with either C16:0 or C18:1 lipid chains and either sulphated or nonsulphated on the reducing end of the chitin backbone, but, unlike Nod factors, they are not O-acetylated (for a comparison of structures, see Fig. S7). Experiments using M. truncatula ROCs expressing the Nup-YC2.1 cameleon showed that 10−8 M sulphated Myc LCOs (a mix of C16:0 and C18:1) were totally unable to activate Ca2+ spiking (Fig. 3). However, as for the S. meliloti Nod factors, a low-level spiking response could be detected when the concentration of sulphated Myc LCOs was increased to 10−5 M (not shown). By contrast, nonsulphated Myc LCOs elicited moderate spiking at 10−7 M (responses in c. 50% of cells) and a low-level spiking could be detected at 10−8 M (Fig. 3). Again, this nonregular spiking is maintained in an nfp mutant background (Fig. S6c,d), suggesting that Myc-LCOs are perceived in ROCs with a lower affinity by the same NFP-independent pathway as short-chain COs. In conclusion, these data indicate that the addition of the sulphate residue and the fatty acid chain to the chitin backbone leads to a significant reduction in NFP-independent Ca2+ spiking activity in ROCs.
Although cameleon-expressing ROCs have proved to be excellent tools to identify and characterize AM-specific signalling molecules, because of the lack of interference with host responses to rhizobial LCOs, it was essential to examine Ca2+ spiking responses elicited in whole plants by short-chain chitin oligomers. Since preliminary experiments had shown no differences in responses to either Nod factor or CO4 when applied to roots from either regenerated transgenic plants or WT composite plants (not shown), we present here a comparison of responses in both WT and nfp backgrounds exclusively using composite plants. A typical spiking profile elicited by 10−9 M S. meliloti Nod factors in root hairs of composite M. truncatula plants expressing 35S:Nup-YC2.1 is shown in Fig. 4(a). As previously demonstrated, this characteristic spiking is totally absent in an nfp mutant background (Ben Amor et al., 2003), and we have confirmed that this is the case even when the Nod factor concentration is increased to 10−5 M (Fig. 4b). On the other hand, in line with the results obtained using ROCs, 10−8 M CO4 elicits a less frequent and less regular Ca2+ spiking profile in root hairs of the whole WT plant (Fig. 4c). Furthermore, the fact that the CO4-induced spiking is maintained in an nfp background (Fig. 4d) confirms that the putative Nod factor receptor is not required for AM-related signal perception in whole plants. In conclusion, the characteristics of the Ca2+ spiking response elicited by short-chain chitin oligomers appear to be identical whether in M. truncatula plants or root organ cultures, whereas spiking responses to LCOs such as S. meliloti Nod factors differ radically in terms of spiking signature, required LCO concentration and NFP dependency. These results argue that COs and sulphated LCOs activate distinct Ca2+-mediated signalling pathways in intact plants.
- Top of page
- Materials and Methods
- Supporting Information
Short-chain chitin oligomers activate an AM-related signalling pathway
Essential steps of host-microbe recognition for both the rhizobial and AM endosymbioses take place before root colonization when the appropriate microbial signals are perceived by root epidermal tissues. This leads to the activation of respective host signal transduction pathways, of which a conserved ‘common SYM’ segment includes both the triggering of intracellular Ca2+ spiking (Oldroyd & Downie, 2006) and its decoding via the essential nuclear-localized calcium and calmodulin-dependent kinase (CCaMK = DMI3). By making use of M. truncatula root organ cultures expressing a nuclear-localized cameleon as a Nod-independent bioassay for AM signals, we provide evidence in this article that short-chain chitin oligosaccharides are able to trigger an AM-related signalling pathway and that the two most active chitin oligomers (CO4 and CO5) are present in AM GSEs at concentrations compatible with the Ca2+ spiking activities of the exudates. Furthermore, major increases in both the CO4/5 concentrations and the associated Ca2+ spiking activities of R. irregularis exudates were observed when the synthetic strigolactone GR24 was included in the germination medium. Besserer et al. (2006, 2008) have shown that the mitochondrial and energy metabolism of AM fungi is rapidly triggered in response to GR24. Therefore, the increase of COs observed here could simply be a consequence of a general metabolic boost. However, the fact that GR24-dependent increases in CO concentrations were significantly higher for the most active CO4 and CO5 compared with the other short-chain COs suggests that this qualitative modification in the exudates corresponds to a change in the physiological status of the AM fungus. By comparison, preliminary experiments failed to reveal any significant modification to short-chain CO concentrations in C. trifolii exudates in response to GR24 treatment (not shown). Genetic experiments have confirmed that the CO-elicited spiking in M. truncatula ROCs is dependent on the common SYM pathway genes MtDMI1 and MtDMI2, and furthermore that inactivation of the MtNFP gene, encoding the LysM RLK essential for Nod factor perception (Arrighi et al., 2006), did not modify the Ca2+ spiking activity of short-chain chitin oligomers. This is consistent with the recent demonstration that the putative Nod factor receptors from Lotus japonicus, NFR1 and NFR5, can directly bind Mesorhizobium loti Nod factors in vitro with a significantly higher affinity compared to the chitin oligomer CO5 (Broghammer et al., 2012). Finally, the discovery that 10−8 M CO4 is equally active in eliciting nuclear Ca2+ spiking on roots of nonlegume hosts such as D. carota (and not on roots of the nonhost A. thaliana) provides additional evidence in favour of the hypothesis that chito-oligosaccharides are novel factors capable of activating an AM-related transduction pathway.
In this context, it is important to underline the fact that Walker et al. (2000) and Oldroyd et al. (2001) were the first to show that short-chain COs could elicit SYM pathway-dependent intracellular Ca2+ spiking in root hairs of pea and M. truncatula seedlings respectively. At the time it was not envisaged that these molecules could be symbiotic fungal signals and the fact that experiments were performed on whole plants made it difficult to discriminate between ‘Nod’ and ‘Myc’ signalling. We show in this article that 10−8 M CO4 can elicit nonregular and NFP-independent spiking in the root epidermis of whole M. truncatula plants as well as in ROCs. The fact that much higher CO4 concentrations (10−4 M) were required to induce Ca2+ spiking in the experiments performed by Oldroyd et al. (2001) on root hairs of 1-d-old seedlings suggests that the sensitivity to CO signals probably depends on various factors, including the stage of root development (Chabaud et al., 2011) and the epidermal cell type (atrichoblasts vs root hairs).
How do plants distinguish between chitin-based pathogenic and symbiotic signals?
Chitinaceous molecules are widespread microbial signals and their perception is essential for plants to discriminate between a variety of biotic stimuli. It is now known that receptors with extracellular LysM motifs are required for the recognition of both rhizobial LCOs in legumes (Limpens et al., 2003; Radutoiu et al., 2003) and chitin-based defence elicitors in model plants such as rice and Arabidopsis (Kaku et al., 2006; Miya et al., 2007). In the latter case, the length of the chitin oligomer appears to be crucial, since receptor affinity drops dramatically if the long-chain octamer is replaced by the short-chain tetramer or pentamer (Shibuya et al., 1996). By contrast, our experiments based on the M. truncatula ROC bioassay have revealed the reverse correlation, with maximal Ca2+ spiking elicited by the chitin tetra/pentamers compared with the longer hexamer and octamer. Interestingly, Liu et al. (2012) have recently shown that activation of the AtCERK1 immune receptor requires long-chain chitin oligomer-induced dimerization, and that this activation is attenuated by the presence of short-chain oligomers such as CO5. Further work will, of course, be necessary to attribute a role for short-chain CO signals in the AM endosymbiosis, unrelated to plant defence. Evidence for the role of LysM-type receptors during root mycorrhization has recently come from RNAi studies using the nonlegume Parasponia (Op den Camp et al., 2011), although it should be underlined that these findings relate to arbuscule formation in the root cortex rather than to the initial stages of root infection. Since the M. truncatula nfp mutant has a WT AM phenotype (Ben Amor et al., 2003), it is conceivable that alternative LysM-type receptor(s) are involved in perceiving short-chain COs in M. truncatula. Detailed phylogenetic analysis of this extensive gene family within M. truncatula (Arrighi et al., 2006) and across plant species (Zhang et al., 2007), coupled with inactivation approaches for candidate genes and the AM-specific ROC bioassay, should now provide the means of identifying potential Myc factor receptors in both legume and nonlegume mycorrhizal plants.
It is also of interest in this context that, despite the presence of short-chain COs in the GSE of the pathogenic hemibiotrophic fungus C. trifolii, Ca2+ spiking was not detected in M. truncatula roots following treatment with the Colletotrichum exudate. Since the addition of exogenous CO4 (10−8 M) was unable to compensate for this lack of response, we deduce that C. trifolii GSEs contain either CO4-inhibitors/competitors, or other components that prevent CO4 activity. Finally, in the case of the hemibiotrophic association between the AM host Oryza sativa and Magnaporthe oryzae, Marcel et al. (2010) have shown that the rice CCaMK homologue to M. truncatula DMI3 is not essential for this pathogenic interaction. This therefore suggests that a SYM pathway-related oscillatory Ca2+ signal is not required to establish this pathogenic fungal–plant association. Together, these findings argue that both the perception of short chitin oligomers and the activation in the root epidermis of nuclear Ca2+ spiking via the SYM pathway are specifically required to set in motion the AM fungal symbiosis. Additional experiments to show the link between CO-induced Ca2+-spiking and effective root colonization are required to confirm this hypothesis.
Roles for different chitin-based signals in the AM symbiosis
After many years of obscurity, the molecular language used by AM fungi to communicate to their plant hosts is finally emerging. Maillet et al. (2011) have identified several species of Myc LCOs present at low concentrations in exudates of AM-colonized carrot roots and R. irregularis GSEs. These Myc LCOs, as is the case for their rhizobial counterparts, are active in stimulating lateral root development (Maillet et al., 2011), and, as a consequence, capable of increasing the root surface available for mycorrhizal colonization. Depending upon the concentration of Myc LCOs, this biological activity is partially or totally dependent on the putative Nod factor receptor NFP (Maillet et al., 2011).
We now show that additional chitinaceous signals of AM origin in the form of nondecorated short-length chitin oligomers are able to activate a Ca2+-mediated signalling pathway in root epidermal tissues, most probably via a distinct LysM-type receptor. Our experiments using M. truncatula ROCs demonstrate that short-chain COs are more active than either sulphated or nonsulphated Myc LCOs in triggering AM-dependent Ca2+ spiking. Conversely, it has been shown that 10−8 M chitin tetraose is unable to stimulate root branching in M. truncatula plants (Oláh et al., 2005) and that sulphated LCOs are more active than nonsulphated LCOs in stimulating lateral root development (Maillet et al., 2011). In this context, a recent article describes a transcriptomic analysis of host gene expression in M. truncatula roots in response to either Nod factors or Myc LCOs (Czaja et al., 2012). Despite the overall low levels of gene induction, the authors demonstrate that Myc LCO-induced gene expression is dependent on the NFP gene. This finding further underlines differences in the mechanism of host perception for AM-generated COs and LCOs. Together, this suggests that AM fungi may use two different chitin-based, signals as compared with a single multifunctional LCO for rhizobial species. Finally, it is striking that irregular Ca2+ spiking in the Medicago epidermis is elicited by AM fungal contact at sites of hyphopodia formation (Chabaud et al., 2011), by all the Gigaspora and Rhizophagus GSEs tested so far (Fig. 1) and also by purified short-chain COs (Fig. 2). It remains to be determined what this irregular spiking signifies, and whether it contributes to the specificity of downstream host cellular responses.
After spore germination in the soil, AM hyphae undergo intensive ramification in the immediate vicinity of host roots, probably due to the perception of secreted strigolactones. This ramification amplifies direct hyphal contact with the root outer surface and the differentiation of the preinfection adhesion structures known as hyphopodia. Mutations in the common SYM signalling pathway of M. truncatula result in failure to elaborate the intracellular prepenetration apparatus (PPA) following hyphopodium formation (Genre et al., 2005) and this presumably leads to infection arrest at the root surface. It is therefore likely that critical fungal–plant signalling takes place at this particular stage of the interaction, and this is supported by the observation of intense Ca2+ spiking within nuclei of atrichoblasts directly contacted by hyphopodia (Chabaud et al., 2011). The findings reported in this paper suggest that simple chitin oligomers (or closely related molecules) may be generated at the fungal–plant interface as part of the two-way preinfection molecular exchange involving strigolactones. From an evolutionary perspective, it is conceivable that the establishment of the dialogue between AM fungi and primitive host plants may have exploited simple products of their chitin metabolism as unsophisticated signals. This is also consistent with the fact that the AM association is both widespread and with no apparent host–fungal specificity. In addition to the triggering of common SYM pathway-dependent nuclear Ca2+ spiking, it will, of course, be important in the future to identify downstream mycorrhization-specific genes activated by short-chain COs. It is also an open question as to whether other important signalling molecules are generated at the fungal–host interface created after hyphopodium contact and adhesion. For example, the presence of surface signals, the secretion of additional effectors, and localized increase in exudate concentration could all contribute to the successful recognition of AM fungi as endosymbionts and their subsequent host-regulated entry into the plant root.
- Top of page
- Materials and Methods
- Supporting Information
We are very grateful to Céline Remblières for transforming A. thaliana with the Nup-YC2.1 cameleon and Antonella Faccio for introducing the same construct into D. carota roots. We would like to thank Eric Samain, Hugues Driguez and Sébastien Fort (CNRS, Grenoble, France), as well as Fabienne Maillet and Jean Dénarié (LIPM, Castanet-Tolosan, France), for kindly providing us with Myc LCOs. Many thanks also to Dr Shibuya for providing us with chitooctaose, and to Elodie Gaulin, Bernard Dumas, Arnaud Bottin and Christophe Jacquet (LRSV, Castanet Tolosan, Toulouse) for fruitful discussions. Research was funded by the Converging Technologies project funded by Regione Piemonte (ref. CIPE-BIOBIT), the Italian National project PRIN 2008, the University of Turin (Ricerca Locale 2008), the French National Research Agency project (ref. ANR-08-BLAN-0029-01) and a joint project financed by the French National Centre Scientific Research (ref. PICS 4267). C.B. and T.R. were supported by PhD fellowships from the French Ministry of Research and Higher Education. This study is part of the TULIP Laboratoire d'Excellence (ANR-10-LABX-41). Confocal microscopy in Toulouse was performed using the Imagery Platform facilities of the Federated Research Institute (FRAIB), and Q Trap mass spectrometry analysis was performed using facilities of the MetaToul Platform, LISBP/INSA, Toulouse.
- Top of page
- Materials and Methods
- Supporting Information
- 2005. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature 435: 824–827. , , .
- 1999. Legume nodulation and mycorrhizae formation; two extremes in host specificity meet. EMBO Journal 18: 281–288. , , .
- 2006. The Medicago truncatula lysin motif-receptor-like kinase gene family includes NFP and new nodule-expressed genes. Plant Physiology 142: 265–279. , , , , , , , , , et al.
- 1988. Early events of vesicular–arbuscular mycorrhiza formation on Ri T-DNA transformed roots. New Phytologist 108: 211–218. , .
- 2003. The NFP locus of Medicago truncatula controls an early step of Nod factor signal transduction upstream of a rapid calcium flux and root hair deformation. Plant Journal 34: 495–506. , , , , , , , , , .
- 2008. GR24, a synthetic analogue of strigolactones, stimulates the mitosis and growth of the arbuscular mycorrhizal fungus Gigaspora rosea by boosting its energy metabolism. Plant Physiology 148: 402–413. , , , , .
- 2006. Strigolactones stimulate arbuscular mycorrhizal fungi by activating mitochondria. PLoS Biology 4: e226. , , , , , , , , , .
- 2001. Agrobacterium rhizogenes-transformed roots of Medicago truncatula for the study of nitrogen-fixing and endomycorrhizal symbiotic associations. Molecular Plant–Microbe Interactions 14: 695–700. , , , , , .
- 2012. Legume receptors perceive the rhizobial lipochitin oligosaccharide signal molecules by direct binding. Proceedings of the National Academy of Sciences, USA 109: 13859–13864. , , , , , , , , , et al.
- 2000. Four genes of Medicago truncatula controlling components of a Nod factor transduction pathway. The Plant Cell 12: 1647–1666. , , , , , , , , , .
- 2011. Arbuscular mycorrhizal hyphopodia and germinated spore exudates trigger Ca2+ spiking in the legume and nonlegume root epidermis. New Phytologist 189: 347–355. , , , , , , , .
- 2002. Targeted inoculation of Medicago truncatula in vitro root cultures reveals MtENOD11 expression during early stages of infection by arbuscular mycorrhizal fungi. New Phytologist 156: 265–273. , , , , .
- 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal 16: 735–743. , .
- 2012. Transcriptional responses towards diffusible signals from symbiotic microbes reveal MtNFP-and MtDMI3-dependent reprogramming of host gene expression by arbuscular mycorrhizal fungal lipochitooligosaccharides. Plant Physiology 159: 1671–1685. , , , , , , , , .
- 1993. Lipo-oligosaccharide nodulation factors: a new class of signalling molecules mediating recognition and morphogenesis. Cell 74: 951–954. , .
- 2007. Lyso-phosphatidylcholine is a signal in the arbuscular mycorrhizal symbiosis. Science 318: 265–268. , , , , , , , , .
- 1996. Calcium spiking in plant root hairs responding to Rhizobium nodulation signals. Cell 85: 673–681. , , .
- 2005. Arbuscular mycorrhizal fungi elicit a novel intracellular apparatus in Medicago truncatula root epidermal cells before infection. The Plant Cell 17: 3489–3499. , , , , .
- 2008. Strigolactone inhibition of shoot branching. Nature 455: 189–194. , , , , , , , , , et al.
- 2006. Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proceedings of the National Academy of Sciences, USA 103: 11086–11091. , , , , , , , .
- 2003. A diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific MtENOD11 expression in roots of Medicago truncatula. Plant Physiology 131: 952–962. , , , , , , .
- 2008. Differential and chaotic calcium signatures in the symbiosis signaling pathway of legumes. Proceedings of the National Academy of Sciences, USA 105: 9823–9828. , , , , , , .
- 2012. A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching. Nature 483: 3–8. , , , , , , , , , .
- 2012. Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level. New Phytologist 193: 970–984. , , , , .
- 2010. Membrane steroid-binding protein 1 induced by a diffusible fungal signal is critical for mycorrhization in Medicago truncatula. New Phytologist 185: 716–733. , , .
- 2003. LysM domain receptor kinases regulating rhizobial Nod factor-induced infection. Science 302: 630–633. , , , , , .
- 2012. Chitin-induced dimerization activates a plant immune receptor. Science 336: 1160–1164. , , , , , , , , , et al.
- 2011. Fungal lipochitooligosaccharide symbiotic signals in arbuscular mycorrhiza. Nature 469: 58–63. , , , , , , , , , et al.
- 2010. Tissue-adapted invasion strategies of the rice blast fungus Magnaporthe oryzae. The Plant Cell 22: 3177–3187. , , , , .
- 2006. Analysis of calcium spiking using a cameleon calcium sensor reveals that nodulation gene expression is regulated by calcium spike number and the developmental status of the cell. Plant Journal 48: 883–894. , , , .
- 2007. CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis. Proceedings of the National Academy of Sciences, USA 104: 19613–19618. , , , , , , , , , .
- 1997. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388: 882–887. , , , , , , .
- 2011. Germinated spore exudates from arbuscular mycorrhizal fungi: molecular and developmental responses in higher land plants and their regulation by ethylene. Molecular Plant–Microbe Interactions 24: 260–270. , .
- 2007. A diffusible signal from arbuscular mycorrhizal fungi elicits a transient cytosolic calcium elevation in host plant cells. Plant Physiology 144: 673–681. , , , , , , .
- 2005. Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway. Plant Journal 44: 195–207. , , , , .
- 2006. Nuclear calcium changes at the core of symbiosis signalling. Current Opinion in Plant Biology 9: 351–357. , .
- 2001. Evidence for structurally negative feedback in the Nod factor signal transduction pathway. Plant Journal 28: 101–109. , , , .
- 2011. LysM-type mycorrhizal receptor recruited for Rhizobium symbiosis in the non-legume Parasponia. Science 331: 909–912. , , , , , , , , , et al.
- 2012. Plant genes related to gibberellin biosynthesis and signaling are differentially regulated during the early stages of AM fungal interactions. Molecular Plant 5: 951–954. , , , , , .
- 2008. Arbuscular mycorrhiza: the mother of plant root endosymbioses. Nature Reviews Microbiology 6: 763–775. .
- 2003. Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases. Nature 425: 585–592. , , , , , , , , , et al.
- 1996. Localization and binding characteristics of a high-affinity binding site for N-acetylchitooligosaccharide elicitor in the plasma membrane from suspension-cultured rice cells suggest a role as a receptor for the elicitor signal at the cell surface. Plant and Cell Physiology 37: 894–898. , , , , , .
- 2001. Oligosaccharide signalling for defence responses in plants. Physiological and Molecular Plant Pathology 59: 223–233. , .
- 2010. Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice. Plant Journal 64: 204–214. , , , , , , , , , et al.
- 2009. A nuclear-targeted cameleon demonstrates intranuclear Ca2+ spiking in Medicago truncatula root hairs in response to rhizobial nodulation factors. Plant Physiology 151: 1197–1206. , , , , , .
- 2009. Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi. Journal of Chemical Biology 2: 191–202. , , .
- 2004. Cytological, genetic, and molecular analysis to characterize compatible and incompatible interactions between Medicago truncatula and Colletotrichum trifolii. Molecular Plant–Microbe Interactions 17: 909–920. , , , , , , , , .
- 2000. Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula. Proceedings of the National Academy of Sciences, USA 97: 13407–13412. , , , , , , , , .
- 2000. Dissection of nodulation signaling using pea mutants defective for calcium spiking induced by Nod factors and chitin oligomers. Proceedings of the National Academy of Sciences, USA 97: 13413–13418. , , .
- 2004. Fungal elicitation of signal transduction-related plant genes precedes mycorrhiza establishment and requires the DMI3 gene in Medicago truncatula. Molecular Plant–Microbe Interactions 17: 1385–1393. , , , , , .
- 2007. Molecular evolution of lysin motif-type receptor-like kinases in plants. Plant Physiology 144: 623–636. , , , , , , , .
- Top of page
- Materials and Methods
- Supporting Information
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.
Fig. S1 Comparison of MS/MS spectra for the CO4 standard and the AM GSE.
Fig. S2 Semi-quantitative analysis of Ca2+ spiking activities of various chitin oligomers.
Fig. S3 Identification of short-chain COs in Rhizophagus irregularis GSEs.
Fig. S4 Effect of GR24 addition on the Ca2+ spiking activity of R. irregularis GSEs.
Fig. S5 Colletotrichum trifolii GSEs do not activate Ca2+ spiking in the M. truncatula root epidermis.
Fig. S6 Nonregular Ca2+ spiking elicited in M. truncatula ROCs by both rhizobial Nod factors and nonsulphated Myc LCOs.
Fig. S7 Structures of COs and LCOs tested in this article.
Table S1 Total number of roots/epidermal cells analysed for each root (ROC) treatment, corresponding to the data presented in Figs 1-3 and S2
Table S2 LC MS/MS retention times for parent ions corresponding to fully acetylated short-chain chitin oligomers (CO3–6) present in both commercial standards and AM GSEs