The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle
Article first published online: 12 FEB 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 1, pages 82–94, April 2013
How to Cite
Garnett, T., Conn, V., Plett, D., Conn, S., Zanghellini, J., Mackenzie, N., Enju, A., Francis, K., Holtham, L., Roessner, U., Boughton, B., Bacic, A., Shirley, N., Rafalski, A., Dhugga, K., Tester, M. and Kaiser, B. N. (2013), The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle. New Phytologist, 198: 82–94. doi: 10.1111/nph.12166
- Issue published online: 25 FEB 2013
- Article first published online: 12 FEB 2013
- Manuscript Accepted: 17 DEC 2012
- Manuscript Received: 16 OCT 2012
- Australian Centre for Plant Functional Genomics
- DuPont Pioneer
- Australian Research Council Linkage. Grant Number: LP0776635
- nitrogen use efficiency (NUE);
- An understanding of nitrate () uptake throughout the lifecycle of plants, and how this process responds to nitrogen (N) availability, is an important step towards the development of plants with improved nitrogen use efficiency (NUE).
- uptake capacity and transcript levels of putative high- and low-affinity transporters (NRTs) were profiled across the lifecycle of dwarf maize (Zea mays) plants grown at reduced and adequate .
- Plants showed major changes in high-affinity uptake capacity across the lifecycle, which varied with changing relative growth rates of roots and shoots. Transcript abundances of putative high-affinity NRTs (predominantly ZmNRT2.1 and ZmNRT2.2) were correlated with two distinct peaks in high-affinity root uptake capacity and also N availability. The reduction in supply during the lifecycle led to a dramatic increase in uptake capacity, which preceded changes in transcript levels of NRTs, suggesting a model with short-term post-translational regulation and longer term transcriptional regulation of uptake capacity.
- These observations offer new insight into the control of uptake by both plant developmental processes and N availability, and identify key control points that may be targeted by future plant improvement programmes to enhance N uptake relative to availability and/or demand.