Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office.


Fig. S1 Multiple sequence alignments of Arabidopsis NIT1–4 and of nitrilases from various organisms.

Fig. S2 Overproduction of GFP-NIT1 in cultured cells and seedlings.

Fig. S3 Silver stained SDS-PAGE and MALDI-MS data of proteins co-immunopurified with GFP-NIT1 from re-suspended P100 fraction.

Fig. S4 Expression of GFP-NIT1 driven by its own promoter in Arabidopsis seedling root.

Fig. S5 Transcript level of NIT1, 2, 3 and 4 in NIT-RNAi plants; correlation of transcript levels with strength of the phenotype.

Fig. S6 Root phenotypes of plants with NIT1–3 downregulated by RNAi.

Fig. S7 Induction of programmed cell death (PCD) in Arabidopsis cells by acetylsalicylic acid.

Fig. S8 NIT1 localization during induction of PCD by acetylsalicylic acid and during aphidicolin-induced replication stress.

Fig. S9 Sequence alignment of FHIT proteins from Arabidopsis thaliana and Homo sapiens.

Fig. S10 Co-expression of NIT1 and FHIT in NIT-RNAi plants and cultured cells overexpressing GFP-NIT1 and expression of CDKA;1 and CDKB1;2 in NIT-RNAi plants.

Fig. S11 Sequence alignment of Rhodococcus rhodochrous J1 nitrilase and Arabidopsis thaliana NIT1.

Methods S1 Detailed materials and methods.

nph12185-sup-0002-VideoS1.avivideo/avi471KVideo S1 Mitosis in Arabidopsis cultured cell expressing GFP-NIT1 under 35S CaMV promoter.
nph12185-sup-0003-VideoS2.avivideo/avi473KVideo S2 Cytokinesis in Arabidopsis cultured cell expressing GFP-NIT1 under 35S CaMV promoter.