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Fig. S1 Six members of the Astralegus sinicus PHT1 gene family.

Fig. S2 Deduced amino acid sequences of AsPT1 and Pht1 family of A. sinicus and legume.

Fig. S3 Hydrophobicity profiles calculated from the deduced amino acid sequences of AsPT1, AsPT2, AsPT3, AsPT4, AsPT5, and AsPT6.

Fig. S4 Putative cis-regulatory elements in the promoters of mycorrhiza-inducible Pht1 transporters and plant inorganic phosphate transporter genes from A. sinicus and other plant species.

Fig. S5 Spatial expression patterns of the Pht1 family genes in mycorrhizal A. sinicus.

Fig. S6 Subcellular localization of Pht1;1 and Pht1;4 transporters.

Fig. S7 Tissue localization analysis of AsPT2, AsPT3 and AsPT5.

Fig. S8 Phosphate transport properties of AsPT5 in yeast.

Fig. S9 Polyphosphate staining of mycorrhizal control, AsPT1 and AsPT4 RNAi roots.

Table S1 Primers used in this study

Table S2 Accession numbers of the Pht1 family transporter

Methods S1 Detailed procedures.

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