Characterization of the sterol 14α-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function
Version of Record online: 27 FEB 2013
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 3, pages 821–835, May 2013
How to Cite
Fan, J., Urban, M., Parker, J. E., Brewer, H. C., Kelly, S. L., Hammond-Kosack, K. E., Fraaije, B. A., Liu, X. and Cools, H. J. (2013), Characterization of the sterol 14α-demethylases of Fusarium graminearum identifies a novel genus-specific CYP51 function. New Phytologist, 198: 821–835. doi: 10.1111/nph.12193
- Issue online: 12 APR 2013
- Version of Record online: 27 FEB 2013
- Manuscript Accepted: 15 JAN 2013
- Manuscript Received: 19 NOV 2012
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Fig. S1 The spilt-marker deletion strategy of FgCYP51 genes in Fusarium graminearum isolate PH-1.
Fig. S2 In vitro transcript levels of ERG genes.
Fig. S3 Relative quantification of actin and β-tubulin gene expression in vitro.
Fig. S4 Relative quantification of gene expression in infected wheat ears.
Fig. S5 Infection of Arabidopsis floral tissue with PH-1 and FgCYP51 gene deletion mutants.
Fig. S6 Pathogenicity of PH-1 and FgCYP51 gene deletion mutants on apple sections.
Fig. S7 Infection of tomato fruits with PH-1 and FgCYP51 gene deletion mutants.
Fig. S9 Alignment of predicted FgCYP51 amino acid sequences.
|nph12193-sup-0002-FigS8.tif||image/tif||6084K||Fig. S8 The proposed sterol biosynthesis pathway in Fusarium graminearum. The sterol pathway based on compounds detected in the wild-type PH-1 and FgCYP51 gene deletion mutants incubated in PDB media for 48 h. The proposed pathway in wild-type PH-1 is shown in solid arrows. The pathway after FgCYP51B deletion is shown with dashed arrows in the dashed box. The two additional sterols, 4,4,14-trimethyl ergosta-trienol and 4,4,14-trimethyl ergosta-dienol, in the dashed box were identified as TMS esters with m/z 510 and m/z 512.|
Table S1 Primers used in this study
Table S2 Concentrations of fungicides used in azole sensitivity testing
Table S3 The Fusarium–Arabidopsis disease scoring system