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nph12193-sup-0001-FigS1-S7-S9.docxWord document5489K

Fig. S1 The spilt-marker deletion strategy of FgCYP51 genes in Fusarium graminearum isolate PH-1.

Fig. S2 In vitro transcript levels of ERG genes.

Fig. S3 Relative quantification of actin and β-tubulin gene expression in vitro.

Fig. S4 Relative quantification of gene expression in infected wheat ears.

Fig. S5 Infection of Arabidopsis floral tissue with PH-1 and FgCYP51 gene deletion mutants.

Fig. S6 Pathogenicity of PH-1 and FgCYP51 gene deletion mutants on apple sections.

Fig. S7 Infection of tomato fruits with PH-1 and FgCYP51 gene deletion mutants.

Fig. S9 Alignment of predicted FgCYP51 amino acid sequences.

nph12193-sup-0002-FigS8.tifimage/tif6084KFig. S8 The proposed sterol biosynthesis pathway in Fusarium graminearum. The sterol pathway based on compounds detected in the wild-type PH-1 and FgCYP51 gene deletion mutants incubated in PDB media for 48 h. The proposed pathway in wild-type PH-1 is shown in solid arrows. The pathway after FgCYP51B deletion is shown with dashed arrows in the dashed box. The two additional sterols, 4,4,14-trimethyl ergosta-trienol and 4,4,14-trimethyl ergosta-dienol, in the dashed box were identified as TMS esters with m/z 510 and m/z 512.
nph12193-sup-0003-TableS1-S3.docxWord document24K

Table S1 Primers used in this study

Table S2 Concentrations of fungicides used in azole sensitivity testing

Table S3 The Fusarium–Arabidopsis disease scoring system