The family of ammonium transporters (AMT) in Sorghum bicolor: two AMT members are induced locally, but not systemically in roots colonized by arbuscular mycorrhizal fungi
Article first published online: 6 MAR 2013
© No claim to original Swiss government works. New Phytologist © 2013 New Phytologist Trust
Volume 198, Issue 3, pages 853–865, May 2013
How to Cite
Koegel, S., Ait Lahmidi, N., Arnould, C., Chatagnier, O., Walder, F., Ineichen, K., Boller, T., Wipf, D., Wiemken, A. and Courty, P.-E. (2013), The family of ammonium transporters (AMT) in Sorghum bicolor: two AMT members are induced locally, but not systemically in roots colonized by arbuscular mycorrhizal fungi. New Phytologist, 198: 853–865. doi: 10.1111/nph.12199
- Issue published online: 12 APR 2013
- Article first published online: 6 MAR 2013
- Manuscript Accepted: 20 JAN 2013
- Manuscript Received: 12 NOV 2012
- Swiss National Science Foundation. Grant Numbers: 130794, PZ00P3_136651
- Burgundy Regional Council. Grant Number: PARI Agrale 8
- ANR TRANSMUT. Grant Number: ANR-10-BLAN-1604-0
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Methods S1 Plant growth conditions, identification and characterization of sorghum AMT transporters, RNA isolation and qRT-PCR, Immunolocalization, isolation of plasma membrane and soluble proteins.
Fig. S1 Colonization rate G. mosseae (A) and G. intraradices (B).
Fig. S2 N content in Sorghum.
Fig. S3 Shoot and root DW (g).
Table S1 AMT isolated and partially characterized in different plant species
Table S2 Composition of Hoagland solutions
Table S3 Characteristics of the S. bicolor AMT gene family
Table S4 Specific primers for real-time quantitative PCR and for full-length gene amplification
Table S5 Amino acid sequence similarity and identity between the AMT of S. bicolor: SbAMT1;1, SbAMT1;2, SbAMT2;1, SbAMT2;2, SbAMT3;1, SbAMT3;2, SbAMT3;3 and SbAMT4
Table S6 Repartition of root DW (%) between the two pots of the split-root experiment
Table S7 Mean and SD of the relative gene expression of the eight SbAMTs and of the glutamine synthase gene
Table S8 Root length colonization (%) in each pot of the split-root experiment